Shen Lingyu, Yan Hanqiu, Li Weihong, Tian Yi, Lin Changying, Liu Baiwei, Wang Yu, Jia Lei, Zhang Daitao, Yang Peng, Wang Quanyi, Gao Zhiyong
Institute for Infectious Diseases and Endemic Diseases Prevention and Control, Beijing Center for Disease Prevention and Control, Beijing, China.
Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Front Microbiol. 2023 Jan 12;13:1073980. doi: 10.3389/fmicb.2022.1073980. eCollection 2022.
To investigate respiratory virus infections in diarrhea cases and identify the risk of respiratory virus transmission through feces.
Fecal specimens were collected from diarrhea cases in enteric disease clinics in Beijing, China, from 2019 to 2020. Cases that tested negative for norovirus, rotavirus, sapovirus, astrovirus, and enteric adenovirus were included in the study. Real-time RT-PCR was used to detect 16 groups of respiratory viruses, and the major viruses were genotyped. Viruses isolation and digestion of clinical specimens and nucleic acid by artificial gastric acid or artificial bile/pancreatic juice were used to evaluate the risk of respiratory virus transmission through feces.
A total of 558 specimens were collected and 47 (8.42%) specimens were detected positive, 40 (13.33%, 40/300) in 2019, and 7 (2.71%, 7/258) in 2020, including 20 (3.58%) for human rhinovirus (HRV), 13 (2.32%) for Bocavirus (BoV), 6 (1.08%) for parainfluenza virus I (PIV), 4 (0.72%) for coronavirus (CoV) OC43, 3 (0.54%) for respiratory syncytial virus (RSV) A, and 1 (0.18%) for both BoV and CoV OC43. Syndrome coronavirus 2 (SARS-CoV-2) and other viruses were not detected in this study. Eight genotypes were identified in the 13 HRV specimens. BoVs 1 and 2 were identified in nine BoV specimens. HRV infectious virions were successfully isolated from 2 clinical specimens and clinical specimens of HRV, RSV, PIV, and CoV could not be detected after 4 h of digestion and their nucleic acid could not be detected after 2 h of digestion by artificial gastric acid or artificial bile/pancreatic juice.
There may be a risk of respiratory virus transmission from diarrhea cases, and interventions against SARS-COV-2 epidemics are also effective for other respiratory viruses.
调查腹泻病例中的呼吸道病毒感染情况，并确定呼吸道病毒通过粪便传播的风险。
收集2019年至2020年中国北京肠道疾病门诊腹泻病例的粪便标本。研究纳入诺如病毒、轮状病毒、札如病毒、星状病毒和肠道腺病毒检测呈阴性的病例。采用实时逆转录聚合酶链反应检测16组呼吸道病毒，并对主要病毒进行基因分型。通过病毒分离以及临床标本经人工胃酸或人工胆汁/胰液消化后的核酸检测，评估呼吸道病毒通过粪便传播的风险。
共收集558份标本，47份（8.42%）标本检测呈阳性，2019年40份（13.33%，40/300），2020年7份（2.71%，7/258），其中人鼻病毒（HRV）20份（3.58%）、博卡病毒（BoV）13份（2.32%）、副流感病毒I型（PIV）6份（1.08%）、冠状病毒（CoV）OC43型4份（0.72%）、呼吸道合胞病毒（RSV）A 3份（0.54%）、BoV和CoV OC43型各1份（0.18%）。本研究未检测到严重急性呼吸综合征冠状病毒2（SARS-CoV-2）及其他病毒。13份HRV标本鉴定出8种基因型。9份BoV标本鉴定出BoV 1型和2型。从2份临床标本中成功分离出HRV感染性病毒粒子，HRV、RSV、PIV和CoV的临床标本经人工胃酸或人工胆汁/胰液消化4小时后未检测到病毒，2小时后未检测到核酸。
腹泻病例可能存在呼吸道病毒传播风险，针对SARS-CoV-2疫情的干预措施对其他呼吸道病毒也有效。
