Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation (FIOCRUZ), Avenida Brasil, 4365 Manguinhos, Rio de Janeiro, RJ, Brazil; Post-Graduate Program in Sanitary Surveillance, National Institute for Quality Control in Health, Oswaldo Cruz Foundation (FIOCRUZ), Avenida Brasil, 4365 Manguinhos, Rio de Janeiro, RJ, Brazil.
Post-Graduate Program in Parasite Biology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation (FIOCRUZ), Avenida Brasil, 4365 Manguinhos, Rio de Janeiro, RJ, Brazil.
Int J Infect Dis. 2020 Jun;95:32-37. doi: 10.1016/j.ijid.2020.03.046. Epub 2020 Apr 3.
This study aimed to verify the frequency, genotypes, and etiological role of Human Bocavirus (HBoV) in younger Amazonian children with either acute gastroenteritis (AGE) or respiratory infections (ARI). The influence of Rotarix™ vaccination and co-infection status was also investigated.
HBoV quantitative polymerase chain reaction (qPCR) testing was done on both fecal and saliva (1468 samples) from 734 children < 5 months old living in the Amazon (Brazil, Guyana, and Venezuela). High and median HBoV viral load samples were used for extraction, nested PCR amplification, and sequencing for genotyping. HBoV mRNA detection was done by reverse transcription following DNA amplification.
The overall HBoV frequencies were 14.2% (69/485; AGE) and 14.1% (35/249; ARI) (p = 0.83). HBoV exclusively infected 4.5% (22/485; AGE) and 4% (10/249) of the Amazonian children (Odds ratios 1.13, 95% confidence interval= 2.42-0.52). HBoV 1 was mainly detected in feces and saliva from AGE children; and HBoV2, from ARI children. HBoV mRNA was detected only in feces. The Rotarix™ vaccination status did not affect the HBoV frequencies.
We suggest that, after entry into the air/oral pathways, HBoV1 continues infecting toward the intestinal tract causing AGE. HBoV2 can be a causative agent of AGE and ARI in younger Amazonian children.
本研究旨在验证人类博卡病毒（HBoV）在急性胃肠炎（AGE）或呼吸道感染（ARI）的年轻亚马逊地区儿童中的频率、基因型和病因作用。还研究了 Rotarix™ 疫苗接种和合并感染状态的影响。
对来自生活在亚马逊地区（巴西、圭亚那和委内瑞拉）的 734 名<5 个月大的儿童的粪便和唾液（1468 份样本）进行 HBoV 定量聚合酶链反应（qPCR）检测。使用高和中位数 HBoV 病毒载量样本进行提取、巢式 PCR 扩增和测序进行基因分型。通过 DNA 扩增后的逆转录进行 HBoV mRNA 检测。
AGE 组和 ARI 组的总 HBoV 频率分别为 14.2%（69/485）和 14.1%（35/249）（p=0.83）。HBoV 仅感染了 4.5%（22/485）和 4%（10/249）的亚马逊地区儿童（优势比 1.13，95%置信区间=2.42-0.52）。AGE 儿童粪便和唾液中主要检测到 HBoV1；ARI 儿童中则主要检测到 HBoV2。仅在粪便中检测到 HBoV mRNA。Rotarix™ 疫苗接种状态不影响 HBoV 频率。
我们认为，HBoV1 进入空气/口腔途径后，继续感染肠道导致 AGE。HBoV2 可能是年轻亚马逊地区儿童 AGE 和 ARI 的致病因子。
