Zhang Xuan, Wang Yonglong, Xu Ying, Babalola Busayo Joshua, Xiang Simin, Ma Jianjun, Su Yun, Fan Yongjun
Faculty of Biological Science and Technology, Baotou Teacher's College, Baotou, China.
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Front Microbiol. 2023 Jan 12;13:1061819. doi: 10.3389/fmicb.2022.1061819. eCollection 2022.
Understanding the underlying mechanisms of microbial community assembly is a fundamental topic in microbial ecology. As an integral part of soil organisms, ectomycorrhizal (EM) fungi play vital roles in ecosystems. is an important pine species in the Helan Mountains in Inner Mongolia, China, with high ecological and economic values. However, studies of EM fungal diversity and mechanisms underlying community assembly on this pine species are limited.
In this study, we investigated EM fungal communities associated with from 45 root samples across three sites in the Helan Mountains using Illumina Miseq sequencing of the fungal rDNA ITS2 region.
A total of 166 EM fungal OTUs belonging to 24 lineages were identified, of which and were the most dominant lineages. Ordination analysis revealed that EM fungal communities were significantly different among the three sites. Site/fungus preference analysis showed that some abundant EM fungal OTUs preferred specific sites. Ecological process analysis implied that dispersal limitation and ecological drift in stochastic processes dominantly determined the community assembly of EM fungi.
Our study indicates that harbors a high EM fungal diversity and highlights the important role of the stochastic process in driving community assembly of mutualistic fungi associated with a single plant species in a semi-arid forest in northwest China.
了解微生物群落组装的潜在机制是微生物生态学的一个基本主题。作为土壤生物的一个组成部分，外生菌根（EM）真菌在生态系统中发挥着至关重要的作用。 是中国内蒙古贺兰山的一种重要松树物种，具有很高的生态和经济价值。然而，关于这种松树物种的EM真菌多样性及其群落组装机制的研究却很有限。
在本研究中，我们使用真菌rDNA ITS2区域的Illumina Miseq测序技术，对来自贺兰山三个地点的45个根系样本中的EM真菌群落进行了调查。
共鉴定出166个属于24个谱系的EM真菌OTU，其中 和 是最主要的谱系。排序分析表明，三个地点的EM真菌群落存在显著差异。地点/真菌偏好分析表明，一些丰富的EM真菌OTU偏好特定的地点。生态过程分析表明，随机过程中的扩散限制和生态漂变主要决定了EM真菌的群落组装。
我们的研究表明， 拥有丰富的EM真菌多样性，并突出了随机过程在中国西北半干旱森林中驱动与单一植物物种相关的互利真菌群落组装的重要作用。
