Fan Y J, Grebenc T, Wei J, Zhao Y L, Yan W, Wang L B
Genet Mol Res. 2016 Sep 2;15(3):gmr8604. doi: 10.4238/gmr.15038604.
We investigated the diversity of ectomycorrhiza associated with the endemic Picea crassifolia in Mount Helan National Nature Reserve in Inner Mongolia, China. Toward this objective, we conducted morphological and molecular identification of ectomycorrhizae in soil cubes taken from pure P. crassifolia stands. Eleven types of ectomycorrhizal (ECM) organisms were separated, briefly described, and identified. Nine morphotypes belonged to the phylum Basidiomycotina [Amphinema byssoides, Cortinarius sp (cf. limonius), Cortinarius vernus, Inocybe cf. nitidiscula, Inocybe sp 1, Sebacina incrustans, Sebacina sp, Suillus luteus, and Piceirhiza tuberculata x Picea crassifolia (comb. Nov.)], and two morphotypes to the phylum Ascomycotina (Cenococcum geophilum and Helvella sp). The diversity of ECM organisms in P. crassifolia was lower than that reported by other studies on spruce or pine forests, or on sporocarp diversity in the high-mountain forests of China. Most of the fungi in the rhizosphere did not correspond to species previously recorded as sporocarps above ground. Here, several new ectomycorrhiza morphotypes are proposed and described. We also confirmed the ectomycorrhizal status of the genus Sebacina (order Sebacinales).
我们对中国内蒙古贺兰山国家级自然保护区内特有植物青海云杉（Picea crassifolia）相关的外生菌根多样性进行了调查。为实现这一目标，我们对取自纯青海云杉林的土块中的外生菌根进行了形态学和分子鉴定。分离出了11种外生菌根（ECM）生物，并对其进行了简要描述和鉴定。9种形态型属于担子菌门[棉絮状双孢菌（Amphinema byssoides）、丝膜菌属（Cortinarius sp (cf. limonius)）、春天丝膜菌（Cortinarius vernus）、近亮盖丝盖伞（Inocybe cf. nitidiscula）、丝盖伞属1（Inocybe sp 1）、硬皮锁瑚菌（Sebacina incrustans）、锁瑚菌属（Sebacina sp）、黄斑牛肝菌（Suillus luteus）以及瘤状云杉根菌×青海云杉（Piceirhiza tuberculata x Picea crassifolia (comb. Nov.)）]，另外两种形态型属于子囊菌门（土生空团菌（Cenococcum geophilum）和皱盖盔菌属（Helvella sp））。青海云杉中外生菌根生物的多样性低于其他关于云杉或松林的研究，以及中国高山森林中孢子果多样性的研究报告。根际中的大多数真菌与先前记录的地上孢子果物种不对应。在此，提出并描述了几种新的外生菌根形态型。我们还确认了锁瑚菌属（Sebacinales目）的外生菌根状态。
