Hoshi Kentaro, Kikuchi Hidezumi, Narita Koji, Fukutoku Yukari, Asari Taka, Miyazawa Kuniaki, Murai Yasuhisa, Sawada Yohei, Tatsuta Tetsuya, Hasui Keisuke, Hiraga Hiroto, Chinda Daisuke, Mikami Tatsuya, Subsomwong Phawinee, Asano Krisana, Nakane Akio, Fukuda Shinsaku, Sakuraba Hirotake
Department of Gastroenterology and Hematology Hirosaki University Graduate School of Medicine Aomori Japan.
Department of Community Medicine Hirosaki University Graduate School of Medicine Aomori Japan.
DEN Open. 2023 Jan 24;3(1):e209. doi: 10.1002/deo2.209. eCollection 2023 Apr.
Gastrointestinal endoscopy increases the risk of bacterial exposure to endoscopists. However, before 2019, most endoscopists did not pay attention to microorganism transmission from patients. This study aimed to investigate the incidence of bacterial exposure to endoscopists' faces during gastrointestinal endoscopic procedures using the bacterial culture method.
This was a single-centered, retrospective study including endoscopists who performed various gastrointestinal endoscopy procedures at the Division of Endoscopy, Hirosaki University Hospital between August 31 and October 6, 2020. Endoscopists wore surgical masks and affixed pre-sterilized films over them. Following the gastrointestinal endoscopic procedures, attached microbes were collected from the endoscopists' surface films using sterilized swabs. Collected microorganisms were cultured on tryptic soy agar and 5% sheep blood agar, and the incidence of bacterial exposure was determined by bacterial culture positivity. Cultured bacteria were identified by gram staining and 16S rRNA gene sequencing.
Bacterial culture positivity was 12.6%, and it was significantly higher in therapeutic than in diagnostic endoscopy. Notably, therapeutic endoscopy increased bacterial culture positivity in colonoscopy, but not in esophagogastroduodenoscopy. , including and , were the most commonly found bacteria in samples identified through 16S rRNA gene sequencing.
The risk of bacterial exposure to the endoscopist's face was increased in colonoscopy treatment procedures. Therefore, endoscopists should be aware of the significant risk of microbial infection from scattering fluid that comes from the endoscopy's working channel.
胃肠内镜检查会增加内镜医师接触细菌的风险。然而，在2019年之前，大多数内镜医师并未关注患者的微生物传播问题。本研究旨在采用细菌培养方法调查胃肠内镜检查过程中内镜医师面部细菌暴露的发生率。
这是一项单中心回顾性研究，纳入了2020年8月31日至10月6日在弘前大学医院内镜科进行各种胃肠内镜检查的内镜医师。内镜医师佩戴外科口罩，并在上面贴上预消毒薄膜。胃肠内镜检查结束后，使用无菌拭子从内镜医师的表面薄膜上收集附着的微生物。收集的微生物在胰蛋白胨大豆琼脂和5%羊血琼脂上培养，通过细菌培养阳性确定细菌暴露的发生率。通过革兰氏染色和16S rRNA基因测序鉴定培养的细菌。
细菌培养阳性率为12.6%，治疗性内镜检查中的阳性率显著高于诊断性内镜检查。值得注意的是，治疗性内镜检查增加了结肠镜检查中的细菌培养阳性率，但在食管胃十二指肠镜检查中未增加。通过16S rRNA基因测序鉴定的样本中，包括[具体细菌名称1]和[具体细菌名称2]在内的[细菌名称]是最常见的细菌。
结肠镜治疗过程中内镜医师面部细菌暴露的风险增加。因此，内镜医师应意识到来自内镜工作通道的散射液导致微生物感染的重大风险。
