Saden-Krehula M, Tajíć M
Department of Chemistry, Faculty of Pharmacy and Biochemistry, University of Zagreb, Yugoslavia.
Pharmazie. 1987 Jul;42(7):471-2.
Unconjugated vitamin D and its metabolites were investigated in the pollen of Pinus nigra Ar. and Pinus sylvestris L. by TLC, HPLC and competitive radiochemical determination of 25-hydroxycholecalciferol (25-OHD3). It was found that vitamin D (D2, D3) was present in the pollen in amounts about 2 micrograms/10 g and 25-OHD3, 24,25-dihydroxycholecalciferol [24,25-(OH)2D3] and 1,25-dihydroxycholecalciferol [1,25-(OH)2D3] between 0.1 and 3 micrograms/10 g of pollen, dependent on pollen species and methods.
通过薄层色谱法（TLC）、高效液相色谱法（HPLC）以及对25-羟基胆钙化醇（25-OHD3）的竞争性放射化学测定，对黑松和欧洲赤松花粉中的游离维生素D及其代谢物进行了研究。结果发现，花粉中维生素D（D2、D3）的含量约为2微克/10克，25-OHD3、24,25-二羟基胆钙化醇[24,25-(OH)2D3]和1,25-二羟基胆钙化醇[1,25-(OH)2D3]的含量在0.1至3微克/10克花粉之间，具体含量取决于花粉种类和测定方法。
