Ma Xueying, Dai Yizhe, Qiu Tiantong, Chen Xin, Xiao Peng, Li Wenhua
Engineering Research Center of Molecular Medicine of Ministry of Education, Key Laboratory of Fujian Molecular Medicine, Key Laboratory of Xiamen Marine and Gene Drugs, Key Laboratory of Precision Medicine and Molecular Diagnosis of Fujian Universities, School of Biomedical Sciences, Huaqiao University, Xiamen, 361021, People's Republic of China.
National and Local Joint Engineering Research Center of Ecological Treatment Technology for Urban Water Pollution, Zhejiang Provincial Key Lab for Water Environment and Marine Biological Resources Protection, Institute for Eco-Environmental Research of Sanyang Wetland, College of Life and Environmental Science, Wenzhou University, 325035, Wenzhou, People's Republic of China.
Environ Sci Pollut Res Int. 2023 Apr;30(16):46248-46256. doi: 10.1007/s11356-023-25584-7. Epub 2023 Jan 30.
Amisulbrom is an oomycete-specific fungicide that was developed by Nissan Chemical Industries Limited. The exposure of developing zebrafish embryo to amisulbrom caused disorders in the visual phototransduction system. However, the potential toxic mechanisms of amisulbrom on retinal development remains unclear. The research purpose of this study was to evaluate the adverse effects of amisulbrom on retinal development in a model organism, the zebrafish. Zebrafish embryos were treated with 0, 0.0075, 0.075, or 0.75 μM amisulbrom from 3 h post-fertilization (hpf) to 72 hpf. Compared with the control group, amisulbrom-treated zebrafish embryos displayed phenotypic microphthalmia, dysregulation of gene transcription levels (alcama, prox1a, sox2, vsx1, rho, bluops, rdops, uvops, and grops) related to the retinal cell layer differentiation, and increased retinal apoptosis. In addition, the content of glutathione and malondialdehyde increased significantly after exposure to amisulbrom. Overall, our data demonstrate the toxicity of amisulbrom to eye development, which will help to assess the potential ecotoxicological impacts posed by amisulbrom to aquatic species.
溴虫腈是一种由日产化学工业株式会社开发的专用于卵菌纲的杀菌剂。发育中的斑马鱼胚胎接触溴虫腈会导致视觉光转导系统紊乱。然而，溴虫腈对视网膜发育的潜在毒性机制仍不清楚。本研究的目的是评估溴虫腈对模式生物斑马鱼视网膜发育的不利影响。斑马鱼胚胎在受精后3小时（hpf）至72 hpf期间用0、0.0075、0.075或0.75 μM溴虫腈处理。与对照组相比，经溴虫腈处理的斑马鱼胚胎表现出小眼症表型、与视网膜细胞层分化相关的基因转录水平（alcama、prox1a、sox2、vsx1、rho、bluops、rdops、uvops和grops）失调，以及视网膜细胞凋亡增加。此外，接触溴虫腈后，谷胱甘肽和丙二醛的含量显著增加。总体而言，我们的数据证明了溴虫腈对眼睛发育的毒性，这将有助于评估溴虫腈对水生物种潜在的生态毒理学影响。
