Engineering Research Center of Molecular Medicine of Ministry of Education, Key Laboratory of Fujian Molecular Medicine, Key Laboratory of Xiamen Marine and Gene Drugs, Key Laboratory of Precision Medicine and Molecular Diagnosis of Fujian Universities, School of Biomedical Sciences, Huaqiao University, Xiamen, 361021, PR China.
Engineering Research Center of Molecular Medicine of Ministry of Education, Key Laboratory of Fujian Molecular Medicine, Key Laboratory of Xiamen Marine and Gene Drugs, Key Laboratory of Precision Medicine and Molecular Diagnosis of Fujian Universities, School of Biomedical Sciences, Huaqiao University, Xiamen, 361021, PR China.
Chemosphere. 2021 Nov;283:131236. doi: 10.1016/j.chemosphere.2021.131236. Epub 2021 Jun 19.
Amisulbrom (AML), a sulfonamide fungicide used to control oomycete diseases, is regarded as a threat to aquatic species. The objective of this study was to evaluate the potential effects of AML on fish using a zebrafish model. Zebrafish embryos were exposed to 0.0075 μM, 0.075 μM, and 0.75 μM AML. AML-treated zebrafish embryos exhibited severe developmental defects, including pericardial edema, blood-clot clustering, increased hatching rates, decreased heart rates, and abnormal hemoglobin distributions. Compared with controls, key marker genes associated with cardiovascular development (i.e., nkx2.5, myh6, myh7, myl7, alas2, hbbe1, hbbe2, and gata1a) were abnormally expressed in response to AML treatment, suggesting that AML might specifically affect cardiovascular development. These results provide a valuable reference for the effects of AML on zebrafish embryos and may help to further clarify the potential risks posed by AML to aquatic ecosystems.
阿米舒必利（AML）是一种用于防治卵菌病害的磺胺类杀菌剂，被认为对水生生物具有威胁。本研究旨在利用斑马鱼模型评估 AML 对鱼类的潜在影响。将斑马鱼胚胎暴露于 0.0075 μM、0.075 μM 和 0.75 μM 的 AML 中。AML 处理的斑马鱼胚胎表现出严重的发育缺陷，包括心包水肿、血凝块聚集、孵化率增加、心率降低以及血红蛋白分布异常。与对照组相比，AML 处理后与心血管发育相关的关键标记基因（即 nkx2.5、myh6、myh7、myl7、alas2、hbbe1、hbbe2 和 gata1a）异常表达，表明 AML 可能会特异性影响心血管发育。这些结果为 AML 对斑马鱼胚胎的影响提供了有价值的参考，并可能有助于进一步阐明 AML 对水生生态系统的潜在风险。
