Blanco-Suárez Elena
Department of Neuroscience, Thomas Jefferson University, Philadelphia, PA, USA.
Methods Mol Biol. 2023;2616:29-38. doi: 10.1007/978-1-0716-2926-0_4.
Photothrombosis is one of the techniques available to reproduce ischemic injuries in animal models. Most of the studies that use photothrombosis resort to this technique as it is highly reproducible and minimally invasive to target cortical brain regions, such as the motor or somatosensory areas. However, this technique can be modified and adapted to virtually target any brain region, including deeper tissue. Here, we describe some variations on the traditional protocol to use the photothrombotic technique to target the longitudinal hippocampal vein in the adult mouse and cause an ischemic injury in the hippocampus.
光血栓形成是在动物模型中重现缺血性损伤的可用技术之一。大多数使用光血栓形成的研究都采用这种技术，因为它具有高度可重复性，并且对大脑皮质区域（如运动或体感区域）进行靶向操作时创伤极小。然而，该技术可以进行修改并适用于几乎任何脑区，包括更深层的组织。在此，我们描述了对传统方案的一些变通方法，以使用光血栓形成技术靶向成年小鼠的海马纵静脉并在海马体中造成缺血性损伤。
