Chang Rudy, Sumbria Rachita K
Department of Biomedical and Pharmaceutical Sciences, School of Pharmacy, Chapman University, Irvine, CA, USA.
Department of Neurology, University of California, Irvine, CA, USA.
Methods Mol Biol. 2023;2616:181-190. doi: 10.1007/978-1-0716-2926-0_14.
Cerebral microhemorrhages are microscopic bleeds in the brain parenchyma and are the pathological substrates of cerebral microbleeds. Clinically and in mouse models, detection of cerebral microhemorrhages involves the use of magnetic resonance imaging and/or postmortem neuropathology techniques including hematoxylin and eosin (H & E) staining to detect extravasated red blood cells and fresh/acute microhemorrhages and Prussian blue staining to detect iron released from extravasated red blood cells and subacute/old microhemorrhages. Here we describe the step-by-step procedure for mouse brain processing and H & E and Prussian blue staining and quantification of acute (H & E-positive) and subacute (Prussian blue-positive) cerebral microhemorrhages in mouse brain tissues.
脑微出血是脑实质中的微观出血，是脑微出血的病理基础。在临床和小鼠模型中，脑微出血的检测涉及使用磁共振成像和/或死后神经病理学技术，包括苏木精和伊红（H&E）染色以检测外渗的红细胞和新鲜/急性微出血，以及普鲁士蓝染色以检测从外渗红细胞释放的铁和亚急性/陈旧性微出血。在这里，我们描述了小鼠脑组织处理以及H&E和普鲁士蓝染色以及小鼠脑组织中急性（H&E阳性）和亚急性（普鲁士蓝阳性）脑微出血定量的分步程序。
