Department of Pharmaceutical Sciences, Center for Blood-Brain Barrier Research, Jerry. H. Hodge School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX, USA.
Methods Mol Biol. 2023;2616:191-203. doi: 10.1007/978-1-0716-2926-0_15.
The blood-brain barrier (BBB) is a dynamic interface responsible for maintaining central nervous system (CNS) homeostasis. An intact BBB protects the brain from undesired compounds and proteins from the blood; however, BBB impairment is involved in various pathological conditions including stroke. In vivo evaluation of BBB integrity in the post-stroke brain is important for investigating stroke-induced CNS pathogenesis and developing CNS-targeted therapeutic agents. In this chapter, we describe both quantitative and morphometric methods and tools to evaluate BBB integrity in vivo. These methods do not require expensive magnetic resonance imaging (MRI) and computed tomography (CT) imaging capabilities and can be conducted in research laboratories with access to a confocal microscope and fluorescence microplate reader.
血脑屏障（BBB）是一个动态界面，负责维持中枢神经系统（CNS）的内稳态。完整的 BBB 可防止大脑受到血液中有害物质和蛋白质的侵害；然而，BBB 损伤与多种病理状况有关，包括中风。在中风后大脑中评估 BBB 的完整性对于研究中风引起的中枢神经系统发病机制和开发中枢神经系统靶向治疗药物非常重要。在本章中，我们描述了定量和形态计量学方法和工具，用于评估体内 BBB 的完整性。这些方法不需要昂贵的磁共振成像（MRI）和计算机断层扫描（CT）成像能力，并且可以在具有共聚焦显微镜和荧光微孔板读数器的研究实验室中进行。
