Syeara Nausheen, Bagchi Sounak, Al Shoyaib Abdullah, Karamyan Serob T, Alamri Faisal F, Karamyan Vardan T
Department of Pharmaceutical Sciences, School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX, USA.
Department of Pharmacology, Faculty of Pharmacy, Yerevan State Medical University, Yerevan, Armenia.
Methods Mol Biol. 2023;2616:345-353. doi: 10.1007/978-1-0716-2926-0_23.
The choice of behavioral tests and their proper execution is critically important for experimental and preclinical therapeutic stroke recovery studies, where improvement of impaired neurological function(s) is the main outcome measure. Two tests that focus on spontaneous motor behaviors of the forelimb during gait and exploratory rearing and are expert recommended for stroke recovery studies in mice are grid-walking and cylinder tasks. Both tests have been widely used in various experimental stroke studies to evaluate acute and chronic motor impairment. To facilitate adoption of these tests and consistency of use between different research laboratories, this chapter describes a simple and rigorous protocol and our schemes to successfully perform both tasks in mice and evaluate motor dysfunction and recovery after stroke. In addition, we provide practical tips to minimize experimental bias and acquire data for analyses.
行为测试的选择及其正确实施对于实验性和临床前治疗性中风恢复研究至关重要，在这些研究中，受损神经功能的改善是主要的结果指标。两项专注于步态和探索性竖立时前肢自发运动行为且被专家推荐用于小鼠中风恢复研究的测试是网格行走和圆筒任务。这两项测试已广泛应用于各种实验性中风研究，以评估急性和慢性运动损伤。为便于采用这些测试并确保不同研究实验室之间使用的一致性，本章描述了一个简单而严谨的方案以及我们在小鼠中成功执行这两项任务并评估中风后运动功能障碍和恢复情况的方案。此外，我们提供实用技巧以尽量减少实验偏差并获取用于分析的数据。
