North Carolina State University, College of Veterinary Medicine, Raleigh, North Carolina, USA.
Equine Vet J. 2023 Nov;55(6):995-1002. doi: 10.1111/evj.13927. Epub 2023 Feb 9.
Intestinal epithelial stem cells (ISC) are responsible for epithelial regeneration and are critical to the intestine's ability to regain barrier function following injury. Evaluating ISC biomarker expression in cases of small intestinal strangulation (SIS) may provide insight into clinical progression.
Intestinal resection margins from cases of SIS were evaluated to determine if (1) evidence of injury could be identified using histomorphometry, (2) ISC biomarker expression was decreased in the proximal resection margin compared to control and distal resection margin, and (3) the ISC biomarker expression was associated with the number of preoperative risk factors negatively related to outcome, post-operative complications, or case outcome.
Retrospective cohort study.
Intestinal samples were obtained intraoperatively from resection margins of adult horses with SIS and horses euthanised for reasons unrelated to colic. Preoperative risk factors negatively related to outcome, post-operative complications, and case outcome were obtained from medical records. Horses were grouped as euthanised intraoperatively, postoperatively, or survived to discharge. Histomorphometry and immunofluorescence were performed to evaluate tissue architecture and ISC and progenitor cell number. Groups were compared using one-way ANOVA. Associations between biomarker expression and the number of preoperative risk factors and post-operative complications negatively related to outcome were determined using linear regression modelling.
Thirty-six cases of SIS were evaluated. Ki67 cell counts were decreased in the proximal (mean = 15.45 cells; 95% CI = 10.27-20.63; SD = 4.17; p = 0.02) and distal resection margins (mean = 15.05; 95% CI = 8.46-21.64; SD = 4.141; p = 0.03) in horses euthanised postoperatively compared to control (mean = 23.62 cells; 95% CI = 19.42-27.83; SD = 5.883). In the distal resection margin, an increase in SOX9 Ki67 cells were associated with a decrease in the total number of preoperative risk factors negatively related to outcome (95% CI = 0.236-1.123; p = 0.008, SE = 0.1393).
Small population size.
Proliferating cell and ISC numbers may be associated with case outcome.
肠上皮干细胞（ISC）负责上皮再生，对于肠道在受伤后恢复屏障功能至关重要。评估小肠绞窄（SIS）病例中的 ISC 生物标志物表达情况，可能有助于了解临床进展。
评估 SIS 病例的肠切除边缘，以确定（1）是否可以通过组织形态计量学识别损伤证据，（2）与对照和远端切除边缘相比，近端切除边缘的 ISC 生物标志物表达减少，以及（3） ISC 生物标志物表达与与结果负相关的术前危险因素数量、术后并发症或病例结果相关。
回顾性队列研究。
从患有 SIS 的成年马的手术切除边缘获得肠样本，并从因与绞痛无关的原因安乐死的马中获得样本。从病历中获得与结果负相关的术前危险因素、术后并发症和病例结果。根据术中、术后安乐死或存活至出院，将马匹分为不同组。进行组织形态计量学和免疫荧光检测，以评估组织结构和 ISC 及祖细胞数量。使用单因素方差分析比较各组。使用线性回归模型确定生物标志物表达与与结果负相关的术前危险因素和术后并发症数量之间的关联。
评估了 36 例 SIS 病例。与对照组相比，术后安乐死的马的近端（平均值=15.45 个细胞；95%置信区间=10.27-20.63；SD=4.17；p=0.02）和远端切除边缘（平均值=15.05 个细胞；95%置信区间=8.46-21.64；SD=4.141；p=0.03）中 Ki67 细胞计数减少。在远端切除边缘，SOX9 Ki67 细胞的增加与与结果负相关的术前危险因素总数的减少相关（95%置信区间=0.236-1.123；p=0.008，SE=0.1393）。
样本量小。
增殖细胞和 ISC 数量可能与病例结果相关。
