Lin Jiafu, Xiang Shengwei, Lv Hua, Wang Tiantian, Rao Yulu, Liu Ling, Yuan Dezhi, Wang Xinrong, Chu Yiwen, Luo Dan, Song Tao
Antibiotics Research and Re-evaluation Key Laboratory of Sichuan Province, Sichuan Industrial Institute of Antibiotics, School of Pharmacy, Chengdu University, 610106 Chengdu, China.
Department of Immunology, School of Basic Medical Sciences, Chengdu Medical College, Chengdu, China.
Int J Biol Macromol. 2023 Apr 15;234:123457. doi: 10.1016/j.ijbiomac.2023.123457. Epub 2023 Jan 28.
The discovery of environmentally friendly enzymes that can convert inexpensive and abundant citrus peel pectin into high value-added product is a potential avenue for the citrus peel application. In this study, a novel PL10-family pectate lyase (pelA) was characterized from marine bacterium Echinicola pacifica. PelA was a Ca dependent pectate lyase whose activity was highest at pH 8 and 40 °C. It was capable of degrading polygalacturonic acid (PGA) and citrus peel pectin (CPP), but not apple peel pectin. Notably, PelA hydrolyzed PGA to high molecular weight polysaccharide (average molecular weight 111.4 kDa). Moreover, PelA was also able to degrade CPP from nine distinct citrus species into polysaccharides (average molecular weight ranging from 84.7 to 539.2 kDa) that showed antimicrobial activity against Staphylococcus epidermidis (88.8 %), Bacillus subtilis (99.8 %), Staphylococcus aureus (92.1 %), Escherichia coli (100.0 %) and Klebsiella pneumoniae (86.4 %). Considering the high market value of pectin in the food industry, PelA's capacity to convert citrus pectin into high molecular weight polysaccharides lays a foundation for its applications.
发现能够将廉价且丰富的柑橘皮果胶转化为高附加值产品的环境友好型酶是柑橘皮应用的一个潜在途径。在本研究中，从海洋细菌太平洋棘海杆菌中鉴定出一种新型的PL10家族果胶酸裂解酶（pelA）。PelA是一种钙依赖性果胶酸裂解酶，其活性在pH 8和40℃时最高。它能够降解聚半乳糖醛酸（PGA）和柑橘皮果胶（CPP），但不能降解苹果皮果胶。值得注意的是，PelA将PGA水解为高分子量多糖（平均分子量111.4 kDa）。此外，PelA还能够将9种不同柑橘品种的CPP降解为多糖（平均分子量在84.7至539.2 kDa之间），这些多糖对表皮葡萄球菌（88.8%）、枯草芽孢杆菌（99.8%）、金黄色葡萄球菌（92.1%）、大肠杆菌（100.0%）和肺炎克雷伯菌（86.4%）表现出抗菌活性。考虑到果胶在食品工业中的高市场价值，PelA将柑橘果胶转化为高分子量多糖的能力为其应用奠定了基础。
