Khakpai Fatemeh, Naseroleslami Maryam, Moheb-Alian Marzieh, Ghanimati Elham, Abdollah-Pour Faezeh, Mousavi-Niri Neda
Department of Physiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Physiol Behav. 2023 May 1;263:114100. doi: 10.1016/j.physbeh.2023.114100. Epub 2023 Jan 28.
Type II diabetes mellitus is a group of metabolic disorders considered chronic hyperglycemia resulting from deficits in insulin secretion or insulin function. This disease usually links with various psychological problems such as anxiety and cognitive dysfunctions. Stevia (Stevia rebaudiana Bertoni) is a natural and healthy substitute sweetener for sugar and artificial sweeteners. It has become essential for human diets and food manufacturers. The aim of this research was to investigate the effects of Stevia and Nano-stevia on the regulation of anxiety and memory processes in male diabetic rats. The elevated plus-maze (EPM) test-retest procedure was used to assess anxiety and memory in male diabetic rats. The findings exhibited that induction of diabetes caused a distorted cellular arrangement in the liver tissue of male rats. On the other hand, intra-gastrically administration of Stevia (1 ml/kg) and nano-Stevia (1 ml/kg) indicated a normal appearance in the liver tissue of male diabetic rats. Moreover, induction of diabetes caused the augmentation of blood glucose, reduction in time spent in%open-arm time (%OAT) on the test day, and enhancement of%OAT on the retest day. Therefore, induction of diabetes in rats produced hyperglycemia, anxiogenic effect, and memory impairment and these responses were reversed by drug treatment. Furthermore, intra-gastrically application of Stevia (1 ml/kg) and nano-Stevia (1 ml/kg) reversed the hyperglycemia, anxiogenic effect, and memory impairment in male diabetic rats. Interestingly, Nano-Stevia exhibited the highest significant response rather than Stevia. In conclusion, the results of this research suggested the beneficial properties of Stevia and particularly Nano-Stevia on inducing anti-diabetic effects, anxiolytic behavior, as well as memory improvement in male diabetic rats.
2型糖尿病是一组代谢紊乱疾病，被认为是由胰岛素分泌不足或胰岛素功能缺陷导致的慢性高血糖症。这种疾病通常与各种心理问题有关，如焦虑和认知功能障碍。甜叶菊（Stevia rebaudiana Bertoni）是一种天然健康的糖和人工甜味剂替代品。它已成为人类饮食和食品制造商的必需品。本研究的目的是调查甜叶菊和纳米甜叶菊对雄性糖尿病大鼠焦虑和记忆过程调节的影响。采用高架十字迷宫（EPM）重测程序评估雄性糖尿病大鼠的焦虑和记忆。研究结果表明，糖尿病的诱导导致雄性大鼠肝脏组织细胞排列紊乱。另一方面，胃内给予甜叶菊（1毫升/千克）和纳米甜叶菊（1毫升/千克）后，雄性糖尿病大鼠肝脏组织外观正常。此外，糖尿病的诱导导致血糖升高，测试日在开放臂时间百分比（%OAT）上花费的时间减少，重测日%OAT增加。因此，大鼠糖尿病的诱导产生了高血糖、致焦虑作用和记忆障碍，而药物治疗可逆转这些反应。此外，胃内应用甜叶菊（1毫升/千克）和纳米甜叶菊（1毫升/千克）可逆转雄性糖尿病大鼠的高血糖、致焦虑作用和记忆障碍。有趣的是，纳米甜叶菊比甜叶菊表现出最高的显著反应。总之，本研究结果表明甜叶菊尤其是纳米甜叶菊在诱导抗糖尿病作用、抗焦虑行为以及改善雄性糖尿病大鼠记忆方面具有有益特性。
