GhMYB102通过调控棉花（陆地棉）中的干旱响应基因和脱落酸生物合成来促进抗旱性。

GhMYB102 promotes drought resistance by regulating drought-responsive genes and ABA biosynthesis in cotton (Gossypium hirsutum L.).

作者信息

Liu Ruida, Shen Yanhui, Wang Minxuan, Liu Ruihua, Cui Ziqian, Li Pengzhen, Wu Qidi, Shen Qian, Chen Jing, Zhang Siping, Liu Shaodong, Ma Huijuan, Pang Chaoyou, Ge Changwei

机构信息

State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, Henan, China; Beijing Lianchuang Seed Science Academy, Longping High-tech Subsidiary, Zhengzhou 450001,Henan, China; Hebei Agricultural University, Stare Key Laboratory of Cotton Biology (Hebei Base), Baoding 071001, Hebei, China; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, China.

State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, Henan, China.

出版信息

Plant Sci. 2023 Apr;329:111608. doi: 10.1016/j.plantsci.2023.111608. Epub 2023 Jan 28.

DOI:10.1016/j.plantsci.2023.111608

PMID:36717027

Abstract

The MYB transcription factor (TF) family is among the largest TF families and plays an important role in plant growth and stress response. However, few studies have investigated the role of the MYB gene in drought resistance in cotton. In this study, we analysed the drought transcriptomic data of cotton and identified that the GhMYB102 gene was significantly upregulated in upland cotton during the early stages of drought stress. Bioinformatics analysis showed that the amino acid sequence encoded by GhMYB102 contained two highly conserved MYB binding domains belonging to R2R3-MYB TFs. GhMYB102 was most closely related to AtMYB102. GhMYB102 is mainly expressed in roots and is induced by abiotic stresses and abscisic acid (ABA); it is localised in the nucleus and has transcriptional activation activity. Silencing of GhMYB102 decreased plant drought resistance. In addition, dual-luciferase assays and yeast single hybridisation analysis showed that GhMYB102 could directly bind the MYB motif elements in the promoter regions of GhNCED1 and GhZAT10. These results indicate that GhMYB102 plays a positive role in drought tolerance by regulating the expression of GhNCED1 and GhZAT10. Thus, GhMYB102 enhances drought resistance by participating in ABA biosynthesis or regulating the expression of drought-responsive genes.

摘要

MYB转录因子（TF）家族是最大的TF家族之一，在植物生长和胁迫响应中发挥重要作用。然而，很少有研究调查MYB基因在棉花抗旱中的作用。在本研究中，我们分析了棉花的干旱转录组数据，发现陆地棉中的GhMYB102基因在干旱胁迫早期显著上调。生物信息学分析表明，GhMYB102编码的氨基酸序列包含两个高度保守的MYB结合结构域，属于R2R3-MYB转录因子。GhMYB102与AtMYB102关系最为密切。GhMYB102主要在根中表达，受非生物胁迫和脱落酸（ABA）诱导；它定位于细胞核并具有转录激活活性。GhMYB102的沉默降低了植物的抗旱性。此外，双荧光素酶测定和酵母单杂交分析表明，GhMYB102可以直接结合GhNCED1和GhZAT10启动子区域中的MYB基序元件。这些结果表明，GhMYB102通过调节GhNCED1和GhZAT10的表达在耐旱性中发挥积极作用。因此，GhMYB102通过参与ABA生物合成或调节干旱响应基因的表达来增强抗旱性。