Radovanović Tijana B, Petrović Tamara G, Gavrilović Branka R, Despotović Svetlana G, Gavrić Jelena P, Kijanović Ana, Mirč Marko, Tomašević Kolarov Nataša, Vukov Tanja, Prokić Marko D
Department of Physiology, Institute for Biological Research "Siniša Stanković", National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, Belgrade, 11060, Serbia.
Department of Evolutionary Biology, Institute for Biological Research "Siniša Stanković", National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, Belgrade, 11060, Serbia.
Front Zool. 2023 Jan 31;20(1):6. doi: 10.1186/s12983-023-00486-z.
Urban development results in habitat destruction, affecting populations of amphibians, the most fragile group of vertebrates. With changes in the environment, these animals become more exposed to light and predators. To enhance their chances of survival, they display plasticity of body coloration. Aside from adaptive benefits, animals exhibiting background matching meet the energetic costs and restrictions of changing body tones. To study the physiological consequences of Hyla arborea tadpole adaptation to background color, we followed oxidative stress parameters after rearing larvae on a constant background (black/white) and after changing the background color.
Larvae cultivated for 20 days on constant substrate color exhibited differences in body coloration but without differences in lipid peroxidation (LPO) concentration between dark and pale individuals, suggesting that coloration investment during this period did not induce higher oxidative damage in darker tadpoles. Prolonged exposure of larvae (37 days) to a dark habitat increased antioxidative system defense and LPO concentrations, compared to animals reared permanently in the white surroundings. The positive correlation of oxidative damage with color intensity of individuals points to the physiological consequences of higher investment in the number of pigment cells necessary for dark pigmentation. In individuals faced with non-matching background and change in body coloration, defense system declined and LPO occurred relative to individuals cultivated in white habitat.
Here, we have pointed to consequences related to background matching and stress that amphibians experienced during chromatic adaptations. Background color change causes a complex physiological response affecting the antioxidative defense parameters. This investigation elucidates the accompanying cost of amphibians' adjustment to an altered environment.
城市发展导致栖息地破坏，影响两栖动物种群，两栖动物是脊椎动物中最脆弱的群体。随着环境变化，这些动物更多地暴露在光照和捕食者面前。为提高生存几率，它们展现出体色可塑性。除了适应性益处外，表现出背景匹配的动物还需承担改变体色的能量成本和限制。为研究树蛙蝌蚪对背景颜色适应的生理后果，我们在恒定背景（黑色/白色）饲养幼虫以及改变背景颜色后，跟踪了氧化应激参数。
在恒定底物颜色上饲养20天的幼虫体色存在差异，但深色和浅色个体之间的脂质过氧化（LPO）浓度没有差异，这表明在此期间的体色投入并未在深色蝌蚪中引发更高的氧化损伤。与长期饲养在白色环境中的动物相比，幼虫长时间（37天）暴露在黑暗栖息地会增加抗氧化系统防御和LPO浓度。氧化损伤与个体颜色强度的正相关表明，为深色色素沉着所需的色素细胞数量投入更多会带来生理后果。与饲养在白色栖息地的个体相比，面对不匹配背景和体色变化的个体，其防御系统下降且出现了LPO。
在此，我们指出了与背景匹配相关的后果以及两栖动物在颜色适应过程中所经历的压力。背景颜色变化会引起影响抗氧化防御参数的复杂生理反应。本研究阐明了两栖动物适应环境变化所伴随的代价。
