基于因子分析和 LC-MS/MS 的谱效关系筛选的抗疲劳活性成分。

Screening of anti-fatigue active ingredients of via spectrum-effect relationship based on factor analysis and LC-MS/MS.

机构信息

Key Laboratory of Forest Plant Ecology, Ministry of Education, Chemical Engineering and Resource Utilization; Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University; Heilongjiang Provincial Key Laboratory of ecological utilization of Forestry-based active substances, Harbin, China.

Department of Chemistry, Division of Science and Technology, University of Education, Lahore, Pakistan.

出版信息

Nat Prod Res. 2023 Nov-Dec;37(24):4144-4155. doi: 10.1080/14786419.2023.2171416. Epub 2023 Jan 30.

DOI:10.1080/14786419.2023.2171416

PMID:36718097

Abstract

contains compounds known to have significant anti-fatigue activity. In recent years, it has received extensive attention because it is efficient. However, its active ingredients on antifatigue effect are still unclear. This study attempts to establish the spectrum-effect relationship of antifatigue activity to screen the effective components. The results showed that the similarity of 15 fingerprints obtained by LC-MS/MS was 0.533-0.992, and the chemical structures of 22 common peaks were identified. The anti-fatigue activity of 15 batches of was characterized by forced swimming test of mice and quantified by CAFI, among which S4, S1 and S5 had better activity. 9 components (caffeic acid, 5-(4--β-D-glucosylferoyl)-quinic acid, (±)13-HODE, isofraxidin, eleutheroside E, syringin, pinoresinol diglucoside or its isomer, 7,8-dihydrodehydrocarbinol alcohol-4-β-D-glucoside, secoisolariciresinol-4--β-D-glucoside) highly related to anti-fatigue activity may be the effective components of .

摘要

包含已知具有显著抗疲劳活性的化合物。近年来，它因其高效而受到广泛关注。然而，其抗疲劳作用的活性成分仍不清楚。本研究试图建立抗疲劳活性的谱效关系，以筛选有效成分。结果表明，通过 LC-MS/MS 得到的 15 个指纹图谱的相似度为 0.533-0.992，鉴定出 22 个共有峰的化学结构。通过小鼠力竭游泳试验和 CAFI 对 15 批的抗疲劳活性进行了特征描述，其中 S4、S1 和 S5 具有更好的活性。9 种成分（咖啡酸、5-(4--β-D-葡萄糖基咖啡酰基）奎宁酸、(±)13-HODE、异嗪皮啶、刺五加苷 E、紫丁香苷、松脂醇二葡萄糖苷或其异构体、7,8-二氢脱氢橙皮醇-4-β-D-葡萄糖苷、松脂素-4--β-D-葡萄糖苷）与抗疲劳活性高度相关，可能是的有效成分。