Fujiati Fujiati, Haryati Haryati, Joharman Joharman, Utami Sabrina Wahda
Department of Biochemistry and Biomolecular, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, South Kalimantan, Indonesia.
Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, South Kalimantan, Indonesia.
Rep Biochem Mol Biol. 2022 Oct;11(3):502-510. doi: 10.52547/rbmb.11.3.502.
(Karamunting) is one of South Kalimantan's biodiversity. In previous research on asthma and coal dust exposure models, nebulization with an ethanol extract of leaves has been shown to reduce inflammatory cells. This study aimed to investigate the anti-inflammatory activity on the stabilization of red blood cell membranes and to identify the chemical compounds present in extracts of leaves.
Anti-inflammatory effect of leaves was evaluated by red blood cell membrane stabilization methods. Nonsteroidal anti-inflammatory sodium diclofenac was used as the reference drug. The content of chemical compounds in the ethanol extract of leaves was identified using Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS).
The results of inhibition of red blood cells membrane lysis showed the n-hexane fraction (concentration 25 ppm), ethyl acetate fraction (concentration 50 ppm), and a fraction of water (concentration 50 ppm) with an inhibition level of 54.5%, 81.8%, 63.6%, respectively. The ethyl acetate fraction showed the highest inhibition of hemolysis. This result is not significantly different from the standard anti-inflammatory sodium diclofenac (90.09%). Oleanonic acid and ursonic acid were two similar metabolites in subfractions ethyl acetate 1, 2, and 3, which may have anti-inflammatory properties.
leaves can have potency as an anti-inflammatory by increasing the red blood cell membrane stability equal to lysosome cells, depending on the concentration.
（卡拉蒙廷）是南加里曼丹的生物多样性之一。在先前关于哮喘和煤尘暴露模型的研究中，用树叶乙醇提取物雾化已显示可减少炎症细胞。本研究旨在探讨其对红细胞膜稳定的抗炎活性，并鉴定树叶提取物中存在的化合物。
通过红细胞膜稳定方法评估树叶的抗炎作用。非甾体抗炎药双氯芬酸钠用作参考药物。使用超高效液相色谱 - 质谱联用仪（UPLC-MS）鉴定树叶乙醇提取物中的化合物含量。
抑制红细胞膜裂解的结果显示，正己烷馏分（浓度25 ppm）、乙酸乙酯馏分（浓度50 ppm）和水馏分（浓度50 ppm）的抑制水平分别为54.5%、81.8%、63.6%。乙酸乙酯馏分显示出最高的溶血抑制率。该结果与标准抗炎药双氯芬酸钠（90.09%）无显著差异。齐墩果酸和熊果酸是乙酸乙酯1、2和3亚馏分中的两种类似代谢物，可能具有抗炎特性。
根据浓度不同，树叶可通过提高红细胞膜稳定性而具有与溶酶体细胞相当的抗炎潜力。
