Department of Biochemistry and Molecular Biology, College of Life Sciences, Beijing Normal University, Gene Engineering Drug and Biotechnology Beijing Key Laboratory, Beijing, China.
PeerJ. 2023 Jan 25;11:e14737. doi: 10.7717/peerj.14737. eCollection 2023.
This study aimed to address on the most important concern of surgeons-whether to completely resect tumor. Urine can indicate early changes associated with physiological or pathophysiological processes. Based on these ideas, we conducted experiments to explore changes in the urine proteome between tumor-bearing mice and tumor-resected mice.
The tumor-bearing mouse model was established with MC38 mouse colon cancer cells, and the mice were divided into the control group, tumor-resected group, and tumor-bearing group. Urine was collected 7 and 30 days after tumor resection. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was used to identify the urine proteome, which was analyzed for differentially expressed proteins and functional annotation.
(1) Seven days after tumor resection, 20 differentially expressed proteins distinguished the tumor-resected group and the tumor-bearing group. The identified biological processes included circadian rhythm, Notch signaling pathway, leukocyte cell-cell adhesion, and heterophilic cell-cell adhesion plasma membrane cell adhesion molecules. (2) Thirty days after tumor resection, 33 differentially expressed proteins distinguished the tumor-resected group and the tumor-bearing group. The identified biological processes included cell adhesion; complement activation, the alternative pathway; the immune system process; and angiogenesis. (3) The difference in the urine proteome between the tumor-resected group and the healthy control group was smaller 30 days after tumor resection.
Changes in the urinary proteome can reflect the complete resection of MC38 tumors.
本研究旨在解决外科医生最关心的问题——是否完全切除肿瘤。尿液可以提示与生理或病理生理过程相关的早期变化。基于这些想法，我们进行了实验，以探索荷瘤小鼠和肿瘤切除小鼠尿液蛋白质组的变化。
使用 MC38 结肠癌细胞建立荷瘤小鼠模型，将小鼠分为对照组、肿瘤切除组和荷瘤组。肿瘤切除后第 7 天和 30 天收集尿液。采用液相色谱-串联质谱（LC-MS/MS）技术鉴定尿液蛋白质组，分析差异表达蛋白并进行功能注释。
（1）肿瘤切除后第 7 天，肿瘤切除组和荷瘤组有 20 个差异表达蛋白。鉴定的生物学过程包括昼夜节律、Notch 信号通路、白细胞细胞间黏附、异嗜性细胞间黏附和质膜细胞黏附分子。（2）肿瘤切除后第 30 天，肿瘤切除组和荷瘤组有 33 个差异表达蛋白。鉴定的生物学过程包括细胞黏附、补体激活的替代途径、免疫系统过程和血管生成。（3）肿瘤切除 30 天后，肿瘤切除组和健康对照组尿液蛋白质组的差异较小。
尿液蛋白质组的变化可以反映 MC38 肿瘤的完全切除。
