Sarwar Shatila, Lin Mao-Chia, Amezaga Carolina, Wei Zhen, Iyayi Etinosa, Polk Haseena, Wang Ruigang, Wang Honghe, Zhang Xinyu
Department of Chemical Engineering, Auburn University, Auburn, AL 36849 USA.
Department of Material Engineering, Auburn University, Auburn, AL 36849 USA.
Adv Compos Hybrid Mater. 2023;6(1):49. doi: 10.1007/s42114-023-00630-7. Epub 2023 Jan 26.
The coronavirus disease 2019 (COVID-19) is a highly contagious and fatal disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In general, the diagnostic tests for COVID-19 are based on the detection of nucleic acid, antibodies, and protein. Among different analytes, the gold standard of the COVID-19 test is the viral nucleic acid detection performed by the quantitative reverse transcription polymerase chain reaction (qRT-PCR) method. However, the gold standard test is time-consuming and requires expensive instrumentation, as well as trained personnel. Herein, we report an ultrasensitive electrochemical biosensor based on zinc sulfide/graphene (ZnS/graphene) nanocomposite for rapid and direct nucleic acid detection of SARS-CoV-2. We demonstrated a simple one-step route for manufacturing ZnS/graphene by employing an ultrafast (90 s) microwave-based non-equilibrium heating approach. The biosensor assay involves the hybridization of target DNA or RNA samples with probes that are immersed into a redox active electrolyte, which are detectable by electrochemical measurements. In this study, we have performed the tests for synthetic DNA samples and, SARS-CoV-2 standard samples. Experimental results revealed that the proposed biosensor could detect low concentrations of all different SARS-CoV-2 samples, using such as S, ORF 1a, and ORF 1b gene sequences as targets. This microwave-synthesized ZnS/graphene-based biosensor could be reliably used as an on-site, real-time, and rapid diagnostic test for COVID-19.
The online version contains supplementary material available at 10.1007/s42114-023-00630-7.
2019年冠状病毒病（COVID-19）是由严重急性呼吸综合征冠状病毒2（SARS-CoV-2）引起的一种高度传染性和致命性疾病。一般来说，COVID-19的诊断测试基于核酸、抗体和蛋白质的检测。在不同的分析物中，COVID-19检测的金标准是通过定量逆转录聚合酶链反应（qRT-PCR）方法进行的病毒核酸检测。然而，金标准测试耗时且需要昂贵的仪器设备以及训练有素的人员。在此，我们报告一种基于硫化锌/石墨烯（ZnS/石墨烯）纳米复合材料的超灵敏电化学生物传感器，用于快速直接检测SARS-CoV-2的核酸。我们展示了一种通过超快（90秒）微波非平衡加热方法制造ZnS/石墨烯的简单一步法。该生物传感器检测方法涉及目标DNA或RNA样本与浸入氧化还原活性电解质中的探针杂交，可通过电化学测量进行检测。在本研究中，我们对合成DNA样本和SARS-CoV-2标准样本进行了测试。实验结果表明，所提出的生物传感器可以检测低浓度的所有不同SARS-CoV-2样本，使用如S、ORF 1a和ORF 1b基因序列作为目标。这种微波合成的基于ZnS/石墨烯的生物传感器可可靠地用作COVID-19的现场、实时和快速诊断测试。
在线版本包含可在10.1007/s42114-023-00630-7获取的补充材料。
