The John Bingham Laboratory, NIAB, 93 Lawrence Weaver Road, Cambridge, UK.
Life Sciences, Univ. of Bristol, Bristol, BS8 1TQ, UK.
Plant Genome. 2024 Mar;17(1):e20288. doi: 10.1002/tpg2.20288. Epub 2023 Jan 31.
Genome-wide introgression and substitution lines have been developed in many plant species, enhancing mapping precision, gene discovery, and the identification and exploitation of variation from wild relatives. Created over multiple generations of crossing and/or backcrossing accompanied by marker-assisted selection, the resulting introgression lines are a fixed genetic resource. In this study we report the development of spring wheat (Triticum aestivum L.) chromosome segment substitution lines (CSSLs) generated to systematically capture genetic variation from tetraploid (T. turgidum ssp. dicoccoides) and diploid (Aegilops tauschii) progenitor species. Generated in a common genetic background over four generations of backcrossing, this is a base resource for the mapping and characterization of wheat progenitor variation. To facilitate further exploitation the final population was genetically characterized using a high-density genotyping array and a range of agronomic and grain traits assessed to demonstrate the potential use of the populations for trait localization in wheat.
在许多植物物种中已经开发出了全基因组渐渗系和替换系，这增强了图谱绘制的精度、基因发现以及从野生近缘种中识别和利用变异的能力。通过多次杂交和/或回交以及标记辅助选择创建的渐渗系是一种固定的遗传资源。在这项研究中，我们报告了春小麦（Triticum aestivum L.）染色体片段替换系（CSSLs）的开发，这些替换系是为了系统地从四倍体（T. turgidum ssp. dicoccoides）和二倍体（Aegilops tauschii）祖先物种中捕获遗传变异而生成的。在经过四代回交的共同遗传背景下生成，这是一个用于小麦祖先变异图谱绘制和特征描述的基础资源。为了促进进一步的开发利用，最后得到的群体使用高密度基因分型阵列进行了遗传特征分析，并评估了一系列农艺和谷物特性，以证明这些群体在小麦性状定位方面的潜在用途。
