Department of Biotechnology, Bilecik Seyh Edebali University, Bilecik, Turkey.
Department of Bioengineering, Bilecik Seyh Edebali University, Bilecik, Turkey.
Crit Rev Anal Chem. 2024;54(7):2422-2434. doi: 10.1080/10408347.2023.2172677. Epub 2023 Jan 31.
Patulin is a low molecular weight mycotoxin and poses a global problem, especially threatening food safety. It is also resistant to processing temperatures and is commonly found in fruits and vegetables. Studies have shown that it has toxic effects on animals and humans and the severity of patulin toxicity depends on the amount ingested. Therefore, the consumption of contaminated products, especially in infants and children, is important. The maximum daily intake of PAT that can be tolerated is found to be 0.4 µg/kg body weight to prevent chronic effects and the maximum residue limits in food samples were given as 50 ng/g (∼320 nM). Conventional methods for the detection of PAT have many disadvantages such as the use of expensive equipment, the need for trained personnel, and complicated sample preparation steps. To this extent, various numbers of research have been conducted on selective and sensitive detection of patulin using biosensor platforms in various media. This review presents an overview of the current literature dealing with the studies to develop patulin-specific aptamer-based biosensors and adapts various immobilization methods to increase the sensor response using different nanomaterials. Furthermore, a comparison of biosensors with conventional methods is presented using analytical performance parameters and their practicality for the detection of patulin.
棒曲霉素是一种低分子量真菌毒素，是一个全球性问题，尤其对食品安全构成威胁。它还能耐受加工温度，通常存在于水果和蔬菜中。研究表明，它对动物和人类具有毒性作用，棒曲霉素毒性的严重程度取决于摄入量。因此，食用受污染的产品很重要，尤其是婴儿和儿童。发现可耐受的 PAT 每日最大摄入量为 0.4μg/kg 体重，以预防慢性影响，食品样本中的最大残留限量规定为 50ng/g（约 320nM）。传统的 PAT 检测方法存在许多缺点，例如使用昂贵的设备、需要经过培训的人员以及复杂的样品制备步骤。在这方面，已经在各种介质中使用生物传感器平台对棒曲霉素的选择性和灵敏检测进行了大量研究。本综述介绍了目前关于开发基于适体的棒曲霉素特异性生物传感器的研究文献，并采用各种固定化方法，利用不同的纳米材料提高传感器的响应。此外，还使用分析性能参数和实际应用对生物传感器与传统方法进行了比较，以检测棒曲霉素。
