Department of Seed Science and Technology, Chaudhary Charan Singh Haryana Agricultural University, Hisar, India.
Agricultural Research Station (S.K.N. Agriculture University, Jobner), Fatehpur Shekhawati, Sikar, Rajasthan, India.
PLoS One. 2023 Jan 31;18(1):e0280962. doi: 10.1371/journal.pone.0280962. eCollection 2023.
Seed priming is a simple and cost effective method to obtain a better plant stand under diverse environmental conditions. The current study was designed to determine the optimal priming duration and water volume for wheat seed. For this experiment, three wheat genotypes with distinct genetic and adaptive backgrounds were chosen. Seeds of each genotype were hydroprimed for 7 durations, i.e. 1, 2, 4, 8, 12, 16, and 20 hours, in three different water volumes, i.e. half, equal, and double volume with respect to seed weight and then surface dried for 1 hour. The control was unprimed (dry) seed. The germination characteristics and seedling vigour potential of hydroprimed seeds were evaluated in the lab by recording several parameters such as germination percentage and speed, seedling growth, and vigour indices at two different temperature levels. The results showed that optimal duration for hydropriming of wheat seed is 12 hours with an equal volume with respect to original seed weight, closely followed by 8 hours with double volume. Reduction in seed performance was observed at 16 and 20 hours priming particularly at double volume treatment. Effect of temperature on seed germination showed improvement in seedling vigour at 25°C when compared to 20°C, although effect on germination percentage was non-significant. Volume of water and priming duration showed significant interactive effects demonstrating that a higher volume can give equivalent results at a shorter duration and vice versa. Another experiment was also conducted to compare the on-farm priming (surface dried seed) with conventional priming (seed re-dried to original moisture) taking 3 potential durations i.e. 8, 12 and 16 hours. Results revealed that both priming methods were statistically at par in terms of germination percentage, while, surface drying resulted in better seedling vigour and speed of germination.
种子引发是一种简单且经济有效的方法，可以在不同的环境条件下获得更好的植物定植。本研究旨在确定小麦种子的最佳引发持续时间和水量。为此实验，选择了三个具有不同遗传和适应背景的小麦基因型。每个基因型的种子在三种不同的水量下分别进行 7 个不同的持续时间的水引发处理，即 1、2、4、8、12、16 和 20 小时，然后表面干燥 1 小时。对照为未引发（干燥）种子。通过记录发芽百分比和速度、幼苗生长和活力指数等参数，在实验室评估水引发种子的发芽特性和幼苗活力潜力，在两个不同的温度水平下进行评估。结果表明，小麦种子水引发的最佳持续时间为 12 小时，与原始种子重量等体积，其次是 8 小时，双体积。在 16 和 20 小时引发时，特别是在双体积处理时，观察到种子性能下降。温度对种子发芽的影响表明，与 20°C 相比，25°C 下幼苗活力提高，尽管对发芽百分比的影响不显著。水体积和引发持续时间表现出显著的交互作用，表明较高的体积可以在较短的持续时间内获得等效的结果，反之亦然。还进行了另一个实验，以比较田间引发（表面干燥种子）与常规引发（将种子重新干燥至原始水分）在 3 个潜在持续时间（8、12 和 16 小时）方面的效果。结果表明，两种引发方法在发芽百分比方面在统计学上是相当的，而表面干燥导致更好的幼苗活力和发芽速度。
