利用 SNEAK PEEC 快速进行双等位基因 CRISPR/Cas9 基因敲入的克隆鉴定。
Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC.
机构信息
Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, NY, 10065, USA.
Institut für Medizinische Physik und Biophysik, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.
出版信息
Sci Rep. 2023 Jan 31;13(1):1719. doi: 10.1038/s41598-023-28732-8.
One of the challenges faced by current CRISPR/Cas9 editing strategies is the difficulty in rapidly selecting clonal populations of biallelically edited cells. Here we present Surface engiNeered fluorEscence Assisted Kit with Protein Epitope Enhanced Capture (SNEAK PEEC), a platform that combines human genome editing with cell-surface display, which enables the direct identification of biallelically edited clones with minimal screening.
目前 CRISPR/Cas9 编辑策略面临的挑战之一是难以快速选择双等位基因编辑细胞的克隆群体。在这里，我们提出了表面工程化荧光辅助试剂盒与蛋白表位增强捕获（SNEAK PEEC），该平台将人类基因组编辑与细胞表面展示相结合，能够直接鉴定双等位基因编辑克隆，筛选工作量最小。
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