Band H, Hochstenbach F, McLean J, Hata S, Krangel M S, Brenner M B
Division of Tumor Virology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115.
Science. 1987 Oct 30;238(4827):682-4. doi: 10.1126/science.3672118.
The T cell receptor (TCR) delta protein is expressed as part of a heterodimer with TCR gamma, in association with the CD3 polypeptides on a subset of functional peripheral blood T lymphocytes, thymocytes, and certain leukemic T cell lines. A monoclonal antibody directed against TCR delta was produced that binds specifically to the surface of several TCR gamma delta cell lines and immunoprecipitates the TCR gamma delta as a heterodimer from Triton X-100 detergent lysates and also immunoprecipitates the TCR delta subunit alone after chain separation. A candidate human TCR delta complementary DNA clone (IDP2 O-240/38), reported in a companion paper, was isolated by the subtractive library approach from a TCR gamma delta cell line. This complementary DNA clone was used to direct the synthesis of a polypeptide that is specifically recognized by the monoclonal antibody to TCR delta. This complementary DNA clone thus corresponds to the gene that encodes the TCR delta subunit.
T细胞受体（TCR）δ蛋白作为与TCRγ组成的异二聚体的一部分表达，与功能性外周血T淋巴细胞、胸腺细胞及某些白血病T细胞系的一部分上的CD3多肽相关联。制备了一种针对TCRδ的单克隆抗体，它能特异性结合几种TCRγδ细胞系的表面，并从Triton X-100去污剂裂解物中以异二聚体形式免疫沉淀TCRγδ，在链分离后还能单独免疫沉淀TCRδ亚基。在一篇配套论文中报道，通过消减文库方法从一个TCRγδ细胞系中分离出了一个候选的人类TCRδ互补DNA克隆（IDP2 O-240/38）。该互补DNA克隆用于指导合成一种能被TCRδ单克隆抗体特异性识别的多肽。因此，这个互补DNA克隆对应于编码TCRδ亚基的基因。
