Hata S, Brenner M B, Krangel M S
Division of Tumor Virology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115.
Science. 1987 Oct 30;238(4827):678-82. doi: 10.1126/science.3499667.
A novel T cell receptor (TCR) subunit termed TCR delta, associated with TCR gamma and CD3 polypeptides, was recently found on a subpopulation of human T lymphocytes. T cell-specific complementary DNA clones present in a human TCR gamma delta T cell complementary DNA library were obtained and characterized in order to identify candidate clones encoding TCR delta. One cross-hybridizing group of clones detected transcripts that are expressed in lymphocytes bearing TCR gamma delta but not in other T lymphocytes and are encoded by genes that are rearranged in TCR gamma delta lymphocytes but deleted in other T lymphocytes. Their sequences indicate homology to the variable, joining, and constant elements of other TCR and immunoglobulin genes. These characteristics, as well as the immunochemical data presented in a companion paper, are strong evidence that the complementary DNA clones encode TCR delta.
最近在人类T淋巴细胞亚群上发现了一种新的T细胞受体（TCR）亚基，称为TCRδ，它与TCRγ和CD3多肽相关。为了鉴定编码TCRδ的候选克隆，从人类TCRγδ T细胞互补DNA文库中获得了存在的T细胞特异性互补DNA克隆并进行了表征。一组交叉杂交的克隆检测到在携带TCRγδ的淋巴细胞中表达但在其他T淋巴细胞中不表达的转录本，这些转录本由在TCRγδ淋巴细胞中重排但在其他T淋巴细胞中缺失的基因编码。它们的序列显示出与其他TCR和免疫球蛋白基因的可变、连接和恒定元件具有同源性。这些特征以及一篇配套论文中给出的免疫化学数据，有力地证明了互补DNA克隆编码TCRδ。
