Wang Nan, Ren Daoyuan, Zhang Li, Han Ning, Zhao Yan, Yang Xingbin
Key Laboratory of Ministry of Education for Medicinal Resource and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, Xi'an, China.
Shaanxi Engineering Laboratory for Food Green Processing and Safety Control, and Shaanxi Key Laboratory for Hazard Factors Assessment in Processing and Storage of Agricultural Products, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, China.
J Sci Food Agric. 2023 May;103(7):3402-3413. doi: 10.1002/jsfa.12477. Epub 2023 Feb 14.
Sheep whey protein (SWP), Fu brick tea polysaccharides (FBTP) and stachyose (STA) have been shown to improve immunity, but little is known about the regulatory effect of SWP, FBTP, STA and their combined formula (CF) on immune function and intestinal metabolism of immunosuppressed mice induced by cyclophosphamide (CTX).
Administration of SWP, FBTP, STA or CF restored the levels of body weight, immune organ index, immune organ morphology, cytokines and immunoglobulins in CTX immunosuppressed mice. Interestingly, CF improved all the mentioned parameters more effective than administration of SWP, FBTP or STA alone. In addition, CF was more effective to increase the levels of intestinal immune-related gene expression than FBTP, SWP or STA alone in immunosuppressed mice, suggesting that CF exhibited excellent intestinal immune regulation function. CF also significantly improved cecal concentrations of short-chain fatty acids of CTX-treated mice. Furthermore, metabolomics analysis demonstrated that CF recovered the levels of 28 metabolites associated with the CTX treatment to the levels of normal mice.
Conclusively, these findings suggested that CF as a functional food combination of SWP, FBTP and STA could promote the immune function against human diseases, which providing theoretical support for the co-ingestion of SWP and functional sugars as a feasible strategy for improving the body immunity in the future. © 2023 Society of Chemical Industry.
羊乳清蛋白（SWP）、茯砖茶多糖（FBTP）和水苏糖（STA）已被证明可提高免疫力，但关于SWP、FBTP、STA及其组合配方（CF）对环磷酰胺（CTX）诱导的免疫抑制小鼠免疫功能和肠道代谢的调节作用知之甚少。
给予SWP、FBTP、STA或CF可恢复CTX免疫抑制小鼠的体重、免疫器官指数、免疫器官形态、细胞因子和免疫球蛋白水平。有趣的是，CF改善所有上述参数的效果比单独给予SWP、FBTP或STA更有效。此外，在免疫抑制小鼠中，CF比单独给予FBTP、SWP或STA更有效地提高肠道免疫相关基因表达水平，表明CF具有优异的肠道免疫调节功能。CF还显著提高了CTX处理小鼠盲肠中短链脂肪酸的浓度。此外，代谢组学分析表明，CF将与CTX处理相关的28种代谢物水平恢复到正常小鼠的水平。
总之，这些发现表明，CF作为SWP、FBTP和STA的功能性食品组合可以促进针对人类疾病的免疫功能，这为SWP与功能性糖共同摄入作为未来提高机体免疫力的可行策略提供了理论支持。© 2023化学工业协会。
