Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.
National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian, Liaoning 116034, China.
Food Funct. 2023 Feb 21;14(4):1989-2007. doi: 10.1039/d2fo02712g.
Ulcerative colitis (UC) is closely associated with the disturbance of gut microbiota. Crude polysaccharide-rich extract from Kishinouye has been proven to alleviate dextran sulfate sodium (DSS)-triggered colitis. However, it remains unclear whether the polysaccharides from (REP) in the extract play a predominant role in ameliorating colitis and whether gut microbiota mediates the beneficial effect of REP. Herein, we aimed to investigate the anti-colitis effects of REP and its mechanisms and to explore the role of REP-modulated gut microbiota in alleviating colitis in mice. Oral REP supplementation ameliorated the symptoms, inflammatory responses, colonic damage and gut microbial dysbiosis in colitic mice. REP significantly enriched SCFA-producing bacteria such as and probiotics such as and restored the level of SCFAs especially butyric acid and propionic acid. Next, we found that transplantation of microbiota from REP-treated mice alleviated DSS-induced acute colitis, evidenced by improved gut barrier integrity and lower inflammation compared with mice receiving microbiota from control mice. Notably, dramatically enriched , and SCFA-producing bacteria including and were found in mice receiving microbiota from the REP-treated donor mice. Lastly, the protective effect of REP supplementation on colitis was abolished in the antibiotic-treated mice. Overall, our findings suggest that REP could alleviate DSS-induced colitis in mice by regulating the imbalance of the microbiome. The polysaccharides of Kishinouye have the potential to be developed into promising prebiotic agents for rectifying dysbiosis of gut microbiota and preventing UC.
溃疡性结肠炎（UC）与肠道微生物群的紊乱密切相关。已证实来自 Kishinouye 的粗多糖丰富提取物可缓解葡聚糖硫酸钠（DSS）引发的结肠炎。然而，尚不清楚提取物中的多糖（REP）是否在缓解结肠炎中起主要作用，以及肠道微生物群是否介导了 REP 的有益作用。在此，我们旨在研究 REP 的抗结肠炎作用及其机制，并探索 REP 调节的肠道微生物群在缓解结肠炎中的作用。口服 REP 补充可改善结肠炎小鼠的症状、炎症反应、结肠损伤和肠道微生物失调。REP 显著富集了产 SCFA 的细菌，如 和益生菌，如 和 ，并恢复了 SCFAs 特别是丁酸和丙酸的水平。接下来，我们发现来自 REP 处理的小鼠的微生物群移植可缓解 DSS 诱导的急性结肠炎，与接受对照小鼠微生物群的小鼠相比，其肠道屏障完整性得到改善，炎症水平降低。值得注意的是，在接受来自 REP 处理供体小鼠的微生物群的小鼠中发现了明显丰富的 、 和产 SCFA 的细菌，包括 和 。最后，在接受抗生素处理的小鼠中，REP 补充对结肠炎的保护作用被消除。总体而言，我们的研究结果表明，REP 通过调节微生物组的失衡可缓解 DSS 诱导的结肠炎。Kishinouye 的多糖具有作为纠正肠道微生物群失调和预防 UC 的有前途的益生元的潜力。
