Cao Yu, Gao Jingzhu, Zhang Lihua, Qin Ningbo, Zhu Beiwei, Xia Xiaodong
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.
National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian, Liaoning 116034, China.
Food Funct. 2021 Oct 19;12(20):10121-10135. doi: 10.1039/d1fo02001c.
Jellyfish skin polysaccharides (JSP) were isolated from Kishinouye and contained 55.11% polysaccharides and 2.26% uronic acid. To examine the anti-inflammatory, antioxidant and immunomodulatory activities of JSP , C57BL/6 mice were induced to develop ulcerative colitis by dextran sulfate sodium (DSS) and the roles of dietary JSP supplementation in modulating colitis were explored. JSP supplementation reduced the symptoms of colitis in mice, increased colon length, protected goblet cells, and improved intestinal epithelial integrity and permeability. JSP modulated oxidative stress and inflammatory responses, which was demonstrated by reduced MPO activity, NO level, and levels of pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 in mice. JSP suppressed NF-κB signaling pathways as evidenced by lower levels of phosphorylated p65 and IKB. Moreover, JSP supplementation enhanced the expression of tight junction proteins and mucins, and modulated the composition of the gut microbiota and the production of short-chain fatty acids. Taken together, these results reveal the anti-inflammatory effect of dietary JSP , suggesting the potential of JSP as a nutritional supplement or adjunct strategy in preventing or ameliorating colitis.
海蜇皮多糖（JSP）从枝螅水母中分离得到，其多糖含量为55.11%，糖醛酸含量为2.26%。为研究JSP的抗炎、抗氧化和免疫调节活性，用葡聚糖硫酸钠（DSS）诱导C57BL/6小鼠患溃疡性结肠炎，并探讨日粮添加JSP对结肠炎的调节作用。添加JSP可减轻小鼠结肠炎症状，增加结肠长度，保护杯状细胞，改善肠上皮完整性和通透性。JSP可调节氧化应激和炎症反应，表现为小鼠髓过氧化物酶（MPO）活性、一氧化氮（NO）水平以及促炎细胞因子（包括肿瘤坏死因子-α、白细胞介素-1β和白细胞介素-6）水平降低。JSP可抑制核因子-κB（NF-κB）信号通路，表现为磷酸化p65和IKB水平降低。此外，添加JSP可增强紧密连接蛋白和黏蛋白的表达，调节肠道微生物群组成和短链脂肪酸的产生。综上所述，这些结果揭示了日粮JSP的抗炎作用，表明JSP作为营养补充剂或辅助策略在预防或改善结肠炎方面具有潜力。
