Life Quality Engineering Interest Group, School of Chemical and Environmental Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, Jiangsu 215123, China.
Food Funct. 2023 Feb 21;14(4):2008-2021. doi: 10.1039/d2fo03327e.
Intestinal permeability is a key factor affecting the bioavailability and physiological efficacy of docosahexaenoic acid (DHA) encapsulated in microcapsules. However, how the DHA microcapsules are transformed and the components absorbed across the small intestinal membrane has seldom been examined previously. In this study, an absorption model based on the permeability of the rat small intestine was established to evaluate the intestinal absorption of DHA microcapsules with five formulations after gastrointestinal digestion . For pure glucose solutions, the apparent permeability coefficient () increased from 5.70 ± 0.60 × 10 cm s at 5 mg mL to 20.25 ± 0.88 × 10 cm s at 30 mg mL and decreased to 15.73 ± 0.91 × 10 cm s at 100 mg mL. The values obtained using the model are comparable to those reported in the human jejunum. For algal oil DHA microcapsules with whey protein as the wall material (A-WP-DHA) after digestion, the of glucose released was 3.81 × 10 cm s with an absorption ratio of 59.55% in the model, significantly lower than that from the porcine casing model. The and absorption ratio varied little among the dialysis models with different molecular weight cut-off values. A similar trend was observed for the absorption of amino acids. However, the absorption ratio (26.6%) was the highest in the model for free fatty acids (FFAs) released from the microcapsules due to the rapid accumulation of compounds on the inner wall of the intestinal sac. In addition, the DHA microcapsules with algal oil as the DHA source (36.40%) exhibited a higher absorption ratio of FFAs than that from tuna oil (14.26%) in the model. The wall material compositions seemed to have little effect on FFA absorption. The present study is practically meaningful for the future formulation of DHA microcapsules with enhanced absorption.
肠通透性是影响包封在微胶囊中的二十二碳六烯酸（DHA）生物利用度和生理功效的关键因素。然而，DHA 微胶囊如何在小肠膜中转化以及吸收的成分很少被之前的研究检验过。在这项研究中，建立了一个基于大鼠小肠通透性的吸收模型，以评估五种配方的 DHA 微胶囊在经过胃肠消化后的肠吸收。对于纯葡萄糖溶液，表观渗透系数（）从 5 毫克/毫升时的 5.70 ± 0.60×10 cm/s 增加到 30 毫克/毫升时的 20.25 ± 0.88×10 cm/s，然后下降到 100 毫克/毫升时的 15.73 ± 0.91×10 cm/s。使用模型获得的值与在人空肠中报道的值相当。对于以乳清蛋白为壁材的藻油 DHA 微胶囊（A-WP-DHA），在消化后，释放的葡萄糖的 为 3.81×10 cm/s，在 模型中的吸收比例为 59.55%，显著低于从猪肠衣模型获得的值。不同分子量截止值的 透析模型中的 和吸收比例变化不大。对于从微胶囊中释放的游离脂肪酸（FFAs），氨基酸也表现出类似的吸收趋势。然而，由于化合物在内肠囊内壁上的快速积累，从微胶囊中释放的游离脂肪酸（FFAs）在 模型中的吸收比例（26.6%）最高。此外，在 模型中，藻油来源的 DHA 微胶囊（36.40%）的 FFAs 吸收比例高于金枪鱼油来源的 DHA 微胶囊（14.26%）。壁材组成似乎对 FFA 吸收影响不大。本研究对未来具有增强吸收的 DHA 微胶囊的配方具有实际意义。
