Tweij Thu-Alfeqar R, Al-Issa Maryam A, Hamed Manar, Khaleq Maysaa Ali Abdul, Jasim Abdullah, R Hadi Najah
DEPARTMENT OF BASIC SCIENCE, FACULTY OF DENTISTRY, UNIVERSITY OF KUFA, AL NAJAF AL-ASHRAF, IRAQ.
FACULTY OF PHARMACY, JABIR IBN HAYYAN MEDICAL UNIVERSITY, AL NAJAF AL-ASHRAF, IRAQ.
Wiad Lek. 2022;75(12):2939-2947. doi: 10.36740/WLek202212108.
The aim: This study aimed to examine the anti-inflammatory, and antiapoptotic effects of erythropoietin against kidney injury inducted by ischemia reperfusion in experimental model.
Materials and methods: 20 male Sprague Dawley rats were randomly divided into 4 equal groups: sham (subject to median laparotomy only), control (subject to 30 minutes ischemia and 2hours reperfusion), vehicle (injected by distilled water and subjected to the same procedure of ischemia reperfusion), erythropoietin group (as in vehicle group but the rats pretreated with 1000 U/kg of erythropoietin). The left kidney and blood specimen were collected. The blood utilized to assess serum creatinine. While kidneys utilized to assessed MCP-1, TLR2, and caspase-3 in addition to histopathological evaluation.
Results: Control and vehicle samples showed that a significant elevation in serum creatinine, TLR2, caspase-3, and MCP-1 as compared with sham group. The histological eval¬uation showed a significant rise in kidney injury scores. Kidneys and blood samples of erythropoietin pretreated rats established histopathological and functional improvement as evidenced via reduced kidney injury scores in addition to the reduction in serum creatinine, as well as there were a significant diminished in caspase-3, MCP-1, and TLR2 levels when compared with control and vehicle groups.
Conclusions: Erythropoietin has renoprotective effect against ischemia and reperfusion, which achieved by decrease the inflammatory response as well as antiapoptotic effect.
本研究旨在探讨促红细胞生成素对实验模型中缺血再灌注诱导的肾损伤的抗炎和抗凋亡作用。
将20只雄性Sprague Dawley大鼠随机分为4组，每组5只：假手术组（仅接受正中剖腹术）、对照组（接受30分钟缺血和2小时再灌注）、溶剂对照组（注射蒸馏水并接受相同的缺血再灌注程序）、促红细胞生成素组（与溶剂对照组相同，但大鼠预先用1000 U/kg促红细胞生成素处理）。收集左肾和血液样本。血液用于评估血清肌酐。肾脏用于评估单核细胞趋化蛋白-1（MCP-1）、Toll样受体2（TLR2）和半胱天冬酶-3（caspase-3），并进行组织病理学评估。
对照组和溶剂对照组样本显示，与假手术组相比，血清肌酐、TLR2、caspase-3和MCP-1显著升高。组织学评估显示肾损伤评分显著升高。促红细胞生成素预处理大鼠的肾脏和血液样本显示组织病理学和功能改善，表现为肾损伤评分降低、血清肌酐降低，与对照组和溶剂对照组相比，caspase-3、MCP-1和TLR2水平也显著降低。
促红细胞生成素对缺血再灌注具有肾脏保护作用，这是通过减少炎症反应和抗凋亡作用实现的。
