Anwar Spogmay, Khan Arif-Ullah, Irshad Nadeem
Riphah Institute of Pharmaceutical Sciences, Riphah International University, Islamabad, Pakistan.
Department of Pharmacy, Quaid-I-Azam University, Islamabad, Pakistan.
Naunyn Schmiedebergs Arch Pharmacol. 2023 Jun;396(6):1309-1324. doi: 10.1007/s00210-023-02412-w. Epub 2023 Feb 1.
Migraine is a devitalizing neurovascular disorder that affects millions of people worldwide. This study was directed against the determination of the effectiveness of carvacrol against migraine. In silico results revealed that carvacrol possesses specific scoring values of - 4.4 to - 6 against selected targets. In vivo studies showed that carvacrol (25-50 mg/Kg) decreased migraine pain by reversing thermal allodynia, mechanical allodynia, number of head-scratching, and light phobicity in rats. Levels of glutathione, glutathione-s-transferase, and catalase enhanced in the cortex and trigeminal nucleus caudalis of the animal's brain tissues, i.e., cortex and trigeminal nucleus caudalis with the use of carvacrol, while a significant decrease in lipid peroxide level was seen. Histopathological evaluation showed improvement in cellular architecture and a decrease in expression of certain inflammatory markers such as tumor necrosis factor-alpha, nuclear factor kappa B, interleukin-18, and prostaglandin E2 validated by enzyme-linked immune sorbent assay, immunohistochemistry, and western blot analysis. This study indicates that carvacrol exhibits binding affinities against different targets involved in migraine pathology and possesses anti-migraine action, mediated through anti-inflammatory and anti-oxidant pathways.
偏头痛是一种使身体衰弱的神经血管疾病，影响着全球数百万人。本研究旨在确定香芹酚对偏头痛的疗效。计算机模拟结果显示，香芹酚对选定靶点具有-4.4至-6的特定评分值。体内研究表明，香芹酚（25-50毫克/千克）通过逆转大鼠的热痛觉过敏、机械性痛觉过敏、抓挠次数和畏光反应，减轻了偏头痛疼痛。使用香芹酚后，动物脑组织的皮质和三叉神经尾核中的谷胱甘肽、谷胱甘肽-S-转移酶和过氧化氢酶水平升高，同时脂质过氧化物水平显著降低。组织病理学评估显示细胞结构有所改善，通过酶联免疫吸附测定、免疫组织化学和蛋白质印迹分析验证，某些炎症标志物如肿瘤坏死因子-α、核因子κB、白细胞介素-18和前列腺素E2的表达减少。本研究表明，香芹酚对偏头痛病理过程中涉及的不同靶点具有结合亲和力，并通过抗炎和抗氧化途径发挥抗偏头痛作用。
