Immunology Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran; Mashhad University of Medical Sciences, Mashhad, Iran.
Neurology Research Center, Kerman University of Medical Sciences, Kerman, Iran; Department of Radiology and Nuclear Medicine, VU University Medical Center, Amsterdam, the Netherlands.
Life Sci. 2019 Feb 15;219:257-263. doi: 10.1016/j.lfs.2018.11.051. Epub 2018 Nov 23.
The inflammatory process is a key step in multiple sclerosis (MS) development. Carvacrol exhibits various anti-inflammatory properties. We aimed to assess the Carvacrol effects on clinical manifestations and production of pro-inflammatory (IFN-γ, IL-6 and IL-17) and anti-inflammatory (TGF-β, IL-4, and IL-10) cytokines in experimental autoimmune encephalomyelitis (EAE) as MS animal model.
EAE mice were treated with 5, 10 mg/kg dose of Carvacrol or vehicle, as the control EAE group, every other day until day-21 post EAE induction. On day22, the leukocyte infiltration within the CNS was estimated using hematoxylin-eosin staining. The cytokine production by splenocytes was determined after in vitro stimulating with myelin oligodendrocyte protein (MOG).
The EAE clinical scores in 5 and 10 mg/kg Carvacrol-treated mice were lower than untreated group (P < 0.001 and P < 0.01, respectively). The amounts of IFN-γ and IL-6 production by splenocytes of 5 and 10 mg/kg Carvacrol-administered mice were lower than control group (P < 0.001, and P < 0.01 for IFN-γ respectively; P ˂ 0.05 for IL-6). Splenocytes of 5 and 10 mg/kg Carvacrol-treated mice produced higher levels of TGF-β than untreated mice (P < 0.001). in splenocytes of 5 mg/kg Carvacrol-treated group the IL-10 production was higher while IL-17 secretion was lower than control group (both with P < 0.01).
Carvacrol exhibits modulatory effects on expression of pro- and anti-inflammatory cytokines. It ameliorates EAE clinical and pathological consequences and therefore its potentials may be considered in treating MS patients.
炎症过程是多发性硬化症（MS）发展的关键步骤。香芹酚具有多种抗炎特性。我们旨在评估香芹酚对实验性自身免疫性脑脊髓炎（EAE）作为 MS 动物模型中临床症状和促炎细胞因子（IFN-γ、IL-6 和 IL-17）和抗炎细胞因子（TGF-β、IL-4 和 IL-10）产生的影响。
EAE 小鼠用 5、10mg/kg 剂量的香芹酚或载体（作为对照 EAE 组），每隔一天治疗，直至 EAE 诱导后第 21 天。在第 22 天，通过苏木精-伊红染色估计中枢神经系统内的白细胞浸润。用髓鞘少突胶质细胞蛋白（MOG）体外刺激后，测定脾细胞的细胞因子产生。
与未治疗组相比，5mg/kg 和 10mg/kg 香芹酚治疗组的 EAE 临床评分较低（P<0.001 和 P<0.01）。5mg/kg 和 10mg/kg 香芹酚给药组脾细胞 IFN-γ和 IL-6 的产生量低于对照组（IFN-γ分别为 P<0.001 和 P<0.01；IL-6 为 P<0.05）。5mg/kg 和 10mg/kg 香芹酚处理组的脾细胞产生的 TGF-β水平高于未处理组（P<0.001）。在 5mg/kg 香芹酚治疗组的脾细胞中，IL-10 的产生增加，而 IL-17 的分泌减少（均为 P<0.01）。
香芹酚对促炎和抗炎细胞因子的表达具有调节作用。它改善了 EAE 的临床和病理后果，因此可以考虑将其用于治疗 MS 患者。
