A. P. Avtsyn Research Institute of Human Morphology, B. V. Petrovsky National Research Centre of Surgery, Moscow, Russia.
Bull Exp Biol Med. 2023 Jan;174(3):385-390. doi: 10.1007/s10517-023-05713-5. Epub 2023 Feb 1.
The dynamics of morphofunctional changes in the thymus during the LPS-induced systemic inflammatory response was assessed in prepubertal male Wistar rats in relationship with the resistance to hypoxia. The systemic inflammatory response was modeled by intraperitoneal administration of E. coli O26:B6 LPS. In histological sections of the thymus, the relative number of thymic bodies and proliferative activity of cells were evaluated. The relative number of CD3CD4, CD3CD8, and CD4CD8 cells in the thymus was determined by flow cytometry. The content of HIF-1α and endotoxin was determined in the blood serum. The expression level of Nfkb mRNA was assessed in the liver. The most pronounced changes in the indicators of the functional state of the thymus were detected 3 and 6 h after LPS administration following the increase in the content of HIF-1α and endotoxin in blood serum and Nfkb mRNA expression in the liver. In the thymus, a decrease in the number of thymic bodies consisting of 3-5 epithelial cells and an increase in the number of bodies consisting of 5 or more cells was observed. In 24 h after LPS administration, the relative number of CD3CD4 and CD3CD8 cells in the thymus decreased. At the same time, the number of Ki-67 cells in the subcapsular zone of the thymus increased 6 and 24 h after LPS administration. These data should be taken into account in the development of approaches to the treatment of infectious and inflammatory diseases in prepubertal children.
在 LPS 诱导的全身炎症反应期间，评估了未成年雄性 Wistar 大鼠胸腺的形态功能变化的动力学与对缺氧的抵抗力之间的关系。通过腹腔内给予大肠杆菌 O26:B6 LPS 来模拟全身炎症反应。在胸腺组织学切片中，评估了胸腺小体的相对数量和细胞的增殖活性。通过流式细胞术确定胸腺中 CD3CD4、CD3CD8 和 CD4CD8 细胞的相对数量。在血清中测定 HIF-1α 和内毒素的含量。在肝脏中评估 Nfkb mRNA 的表达水平。在 LPS 给药后 3 和 6 小时，检测到与血清中 HIF-1α 和内毒素含量增加以及肝脏中 Nfkb mRNA 表达增加相关的胸腺功能状态指标的最明显变化。在胸腺中，观察到由 3-5 个上皮细胞组成的胸腺小体数量减少，而由 5 个或更多细胞组成的小体数量增加。在 LPS 给药后 24 小时，胸腺中 CD3CD4 和 CD3CD8 细胞的相对数量减少。同时，LPS 给药后 6 和 24 小时，胸腺被膜下区的 Ki-67 细胞数量增加。在制定未成年儿童感染和炎症性疾病治疗方法时应考虑这些数据。
