Klaudia Chrząstek, Alina Wieliczko
Department of Epizootiology and Clinic of Bird and Exotic Animals, The Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Pl. Grunwaldzki 45, 50-366, Wrocław, Poland.
Vet Res Commun. 2015 Mar;39(1):53-60. doi: 10.1007/s11259-015-9632-7. Epub 2015 Feb 10.
This study aimed to investigate the influence of enrofloxacin, florfenicol, ceftiofur and E. coli LPS interaction on T and B subsets in thymus and spleen of newly-hatched chicks. A 126, 1-day-old chicks were administered enrofloxacin, florfenicol or ceftiofur in recommended doses according to the currently treatment schedule advises. E .coli LPS was given intravenously once at the dose of 200 μg kg(-1) BW on the 2nd day of experiment (d. e.). On the 6th and the 14th d. e. thymus and spleens were subjected to flow cytometry investigation.The most significant changes were demonstrated in spleen. The antibiotics administration decreased the percentage of B and T cells subset. Moreover, this suppressive effect was enhanced by E. coli LPS administration. On the 6th d. e. the percentage of CD3(+)TCRγδ(-), CD3(+)TCRγδ(+), CD4(+)CD8(-), CD4(-)CD8(+) decreased significantly after ceftiofur and LPS treatment. A lower percentage of CD3(+)TCRγδ(-), CD4(-)CD8(+) and CD3(+)TCRγδ(+) was observed in enrofloxacine and LPS treated group. The decrease percentage of CD3(+)TCRγδ(+)cells and Bu-1(+) was found after florfenicol and LPS treatment. On the 14th d. e. a decreased percentage of CD4(+)CD8(-) and increased percentage of CD4(-)CD8(+) cells was shown in ceftiofur or enrofloxacine and LPS treated groups. In addition decreased percentage of CD3(+)TCRγδ(+) was found in all antibiotic and LPS treated groups.In this study, it was shown that enrofloxacine, florfenicol, ceftiofur treatment may change the proportions among lymphocytes subset and might have an impact on the immune response to bacterial endotoxins in chicks.
本研究旨在探讨恩诺沙星、氟苯尼考、头孢噻呋与大肠杆菌脂多糖（LPS）相互作用对新孵出雏鸡胸腺和脾脏中T细胞和B细胞亚群的影响。将126只1日龄雏鸡按照当前治疗方案建议的推荐剂量给予恩诺沙星、氟苯尼考或头孢噻呋。在实验第2天（d.e.），以200μg kg(-1)体重的剂量静脉注射一次大肠杆菌LPS。在实验第6天和第14天，对胸腺和脾脏进行流式细胞术检测。脾脏中出现了最显著的变化。抗生素给药降低了B细胞和T细胞亚群的百分比。此外，大肠杆菌LPS给药增强了这种抑制作用。在实验第6天，头孢噻呋和LPS处理后，CD3(+)TCRγδ(-)、CD3(+)TCRγδ(+)、CD4(+)CD8(-)、CD4(-)CD8(+)的百分比显著降低。在恩诺沙星和LPS处理组中，观察到CD3(+)TCRγδ(-)、CD4(-)CD8(+)和CD3(+)TCRγδ(+)的百分比更低。氟苯尼考和LPS处理后，发现CD3(+)TCRγδ(+)细胞和Bu-1(+)的百分比降低。在实验第14天，头孢噻呋或恩诺沙星与LPS处理组中，CD4(+)CD8(-)的百分比降低，CD4(-)CD8(+)细胞的百分比增加。此外，在所有抗生素与LPS处理组中，均发现CD3(+)TCRγδ(+)的百分比降低。本研究表明，恩诺沙星、氟苯尼考、头孢噻呋处理可能会改变淋巴细胞亚群之间的比例，并可能对雏鸡对细菌内毒素的免疫反应产生影响。
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