Department of Food and Nutritional Sciences, University of Reading, Harry Nursten Building, Pepper Lane, Whiteknights, Reading RG6 6DZ, UK.
BENEO-Institute, BENEO GmbH, Wormser Str. 11, 67283 Obrigheim, Germany.
J Appl Microbiol. 2023 Feb 16;134(2). doi: 10.1093/jambio/lxac069.
In this study, we explored the effects that the prebiotic inulin-type fructans, and prebiotic candidates: 2'fucosyllactose and β-glucan from barley, singular and in combination had on microbial load, microbiome profile, and short-chain fatty acid production. This was carried out as a prescreening tool to determine combinations that could be taken forward for use in a human intervention trial.
Effects of inulin-type fructans, 2'fucosyllactose and β-glucan from barley in singular and combination on microbial load and profile and short-chain fatty acid production (SCFA) was conducted using in vitro batch culture fermentation over 48 h. Changes in microbial load and profile were assessed by fluorescence in situ hybridization flow cytometry (FISH-FLOW) and 16S rRNA sequencing, and changes in SCFA via gas chromatography. All substrates generated changes in microbial load and profile, achieving peak microbial load at 8 h fermentation with the largest changes in profile across all substrates in Bifidobacterium (Q < 0.05). This coincided with significant increases in acetate observed throughout fermentation (Q < 0.05). In comparison to sole supplementation combinations of oligofructose, β-glucan and 2'fuscosyllactose induced significant increases in both propionate and butyrate producing bacteria (Roseburia and Faecalibacterium praunitzii), and concentrations of propionate and butyrate, the latter being maintained until the end of fermentation (all Q < 0.05).
Combinations of oligofructose, with β-glucan and 2'fucosyllactose induced selective changes in microbial combination and SCFA namely Roseburia, F. praunitzii, propionate and butyrate compared to sole supplementation.
在这项研究中，我们探讨了益生元菊糖型果聚糖，以及益生元候选物：2'-岩藻糖基乳糖和大麦β-葡聚糖，单独和组合使用对微生物负荷、微生物组谱和短链脂肪酸（SCFA）产生的影响。这是作为一种筛选工具，以确定可以用于人体干预试验的组合。
在 48 小时的体外批量培养发酵中，单独和组合使用菊糖型果聚糖、2'-岩藻糖基乳糖和大麦β-葡聚糖对微生物负荷和谱以及短链脂肪酸（SCFA）产生的影响。通过荧光原位杂交流式细胞术（FISH-FLOW）和 16S rRNA 测序评估微生物负荷和谱的变化，通过气相色谱评估 SCFA 的变化。所有底物都引起了微生物负荷和谱的变化，在 8 小时发酵时达到了微生物负荷的峰值，所有底物中双歧杆菌的谱变化最大（Q<0.05）。这与整个发酵过程中观察到的乙酸盐显著增加相一致（Q<0.05）。与单独补充寡果糖、β-葡聚糖和 2'-岩藻糖基乳糖相比，组合补充这些物质显著增加了丙酸和丁酸产生菌（Roseburia 和 Faecalibacterium praunitzii），以及丙酸和丁酸的浓度，后者一直维持到发酵结束（所有 Q<0.05）。
寡果糖与β-葡聚糖和 2'-岩藻糖基乳糖的组合与单独补充相比，诱导了微生物组合和 SCFA 的选择性变化，特别是 Roseburia、F. praunitzii、丙酸和丁酸。
