School of Public Health, the key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, China.
State Key Laboratory of Pathogens and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100010, China.
J Appl Microbiol. 2023 Feb 16;134(2). doi: 10.1093/jambio/lxac068.
Human skin is the first barrier against pathogens and environmental hazards and the highest contact frequency occurs with the hands. Environmental and personal metabolic factors may affect skin microbes. This study was conducted to clarify the diversity in the skin microbial community that was mainly due to individual skin metabolites rather than lifestyle and environmental factors.
Skin microbiota samples were collected from 11 volunteers who met similar lifestyle inclusion criteria. The V3-V4 region of the 16S rRNA gene was amplified. After library construction and sequencing, we compared the composition and diversity of the hand skin microbiota in different sexes and BMI groups with bioinformation analysis. The whole sequence data were annotated as 42 phyla, 538 families, and 1215 genera. Four dominant phyla accounted for 97% of the total including Actinobacteriota (50.18%), Firmicutes (23.85%), Proteobacteria (21.64%) and Bacteroidota (2.05%). The genera that were detected in all subjects with high relative abundance were Cutibacterium, Staphylococcus, Corynebacterium, Streptococcus, Lawsonella, Enhydrobacter, Escherichia-Shigella, Asaia and Micrococcus.
The diversity and richness of the microbiota of male hand skin in our study was higher than that of females. Interestingly, Cutibacterium, Staphylococcus, and Corynebacterium might serve as important skin microbiota to distinguish sexes.
人体皮肤是抵御病原体和环境危害的第一道屏障，而手与外界的接触频率最高。环境和个人代谢因素可能会影响皮肤微生物群。本研究旨在阐明皮肤微生物群落的多样性，主要归因于个体皮肤代谢物，而不是生活方式和环境因素。
从符合相似生活方式纳入标准的 11 名志愿者身上采集了皮肤微生物样本。扩增了 16S rRNA 基因的 V3-V4 区。在文库构建和测序后，我们通过生物信息分析比较了不同性别和 BMI 组手部皮肤微生物群落的组成和多样性。全序列数据被注释为 42 个门、538 个科和 1215 个属。四个主要的门占了总序列的 97%，包括放线菌门（50.18%）、厚壁菌门（23.85%）、变形菌门（21.64%）和拟杆菌门（2.05%）。在所有受试者中检测到的高相对丰度的属包括葡萄球菌属、葡萄球菌属、棒状杆菌属、链球菌属、劳森菌属、内水杆菌属、大肠杆菌-志贺菌属、亚洲菌属和微球菌属。
本研究中男性手部皮肤的微生物多样性和丰富度高于女性。有趣的是，葡萄球菌属、葡萄球菌属和棒状杆菌属可能是区分性别的重要皮肤微生物群。
