Departments of General Surgery, Changzhou No.2 People's Hospital, Nanjing Medical University, Changzhou, 213000, China.
Departments of Gastrointestinal Surgery, Jingzhou Central Hospital, Second Clinical Medical College, Yangtze University, Jingzhou, 434000, China.
Biochem Biophys Res Commun. 2023 Mar 12;648:21-27. doi: 10.1016/j.bbrc.2023.01.061. Epub 2023 Jan 20.
Pancreatic cancer has a particularly poor prognosis compared to other tumors. The peculiar hyperinsulin microenvironment of the pancreas is formed due to the endocrine secretion of islets in the pancreas. This study focused on the effect of insulin on the migration and proliferation of pancreatic cancer cells and its molecular mechanisms. We found that insulin promotes the proliferation and migration of pancreatic cancer cells. At the same time, it can up-regulate the expression of PLK1 in pancreatic cancer cells. Knocking down the expression of PLK1 in pancreatic cancer cells can inhibit the effect of insulin on the biological behavior of pancreatic cancer. In addition, we found that insulin activates the PI3K/AKT pathway in pancreatic cancer cells, and that inhibition of this pathway suppresses PLK1 expression. The PI3K/AKT inhibitor LY294002 inhibits the effects of insulin on the proliferation of pancreatic cancer cells. This study shows that insulin up-regulates PLK1 expression in pancreatic cancer cells via the PI3K/AKT pathway, which in this way enhances the migration and proliferation of pancreatic cancer cells. This may be one of the important reasons for the poor prognosis of pancreatic cancer.
与其他肿瘤相比，胰腺癌的预后尤其差。胰腺独特的高胰岛素微环境是由于胰腺胰岛的内分泌分泌形成的。本研究重点研究了胰岛素对胰腺癌细胞迁移和增殖的影响及其分子机制。我们发现胰岛素促进了胰腺癌细胞的增殖和迁移。同时，它可以上调胰腺癌细胞中 PLK1 的表达。敲低胰腺癌细胞中 PLK1 的表达可以抑制胰岛素对胰腺癌细胞生物学行为的影响。此外，我们发现胰岛素激活了胰腺癌细胞中的 PI3K/AKT 通路，而抑制该通路可以抑制 PLK1 的表达。PI3K/AKT 抑制剂 LY294002 抑制了胰岛素对胰腺癌细胞增殖的作用。这项研究表明，胰岛素通过 PI3K/AKT 通路上调胰腺癌细胞中 PLK1 的表达，从而增强了胰腺癌细胞的迁移和增殖。这可能是胰腺癌预后不良的重要原因之一。
