College of Food Science and Engineering, Northwest A&F University, Shaanxi 712100, China.
Ulanqab Vocational College, Ulanqab 012000, China.
Meat Sci. 2023 May;199:109121. doi: 10.1016/j.meatsci.2023.109121. Epub 2023 Jan 19.
Insights into changes in microorganisms and metabolites in irradiated marbled beef may help elucidate the beneficial effects of irradiation on prolonging the shelf life of meat. In this study, 16S rRNA gene sequencing, ultra-high-performance liquid chromatography-tandem mass spectrometry, and Pearson's correlation analyses were conducted to detect key microorganisms, core metabolites, and potential correlation between the microbiome and metabolome in marbling beef. Microbiome analysis showed that irradiation effectively eradicated the spoilage bacterium Leuconostoc and reduced the proportions of Carnobacterium and Lactobacillus in marbled beef. Additionally, results of metabolomic analysis involving irradiated marbled beef revealed that metabolites with significant differences were mainly organic acids and their derivatives, lipids, and lipid-like molecules, including six core metabolites. Furthermore, a significant correlation between key bacteria and metabolites was observed. Carnobacterium, Lactobacillus, and Leuconostoc affected the accumulation of core metabolites in irradiated marbled beef by influencing amino acid and lipid metabolism. Characterization of the microbiota and metabolites, as well as clarification of their correlation, can contribute to a better understanding of the mechanisms whereby irradiation helps maintain meat quality.
辐照大理石纹牛肉中微生物和代谢物变化的研究进展可能有助于阐明辐照对延长肉类货架期的有益作用。本研究采用 16S rRNA 基因测序、超高效液相色谱-串联质谱和 Pearson 相关性分析，检测大理石纹牛肉中关键微生物、核心代谢物及微生物组与代谢组之间的潜在相关性。微生物组分析表明，辐照可有效杀灭腐败菌肠膜明串珠菌，降低大理石纹牛肉中片球菌和乳杆菌的比例。此外，辐照大理石纹牛肉的代谢组学分析结果表明，差异显著的代谢物主要为有机酸及其衍生物、脂质和类脂分子，包括 6 种核心代谢物。此外，还观察到关键细菌和代谢物之间存在显著相关性。肉杆菌、乳杆菌和肠膜明串珠菌通过影响氨基酸和脂质代谢，影响辐照大理石纹牛肉中核心代谢物的积累。对微生物群和代谢物的特征进行描述，并阐明它们之间的相关性，可以帮助更好地理解辐照有助于维持肉类质量的机制。
