Zhang Ju, Zhang Qian, Fan Jianfeng, Yu Jiangtao, Li Kui, Bai Junqing
College of Food Science and Engineering, Northwest A&F University, Shaanxi 712100, China.
Ulanqab Vocational College, Ulanqab 012000, China.
Food Chem X. 2023 Feb 26;17:100617. doi: 10.1016/j.fochx.2023.100617. eCollection 2023 Mar 30.
Electron beam irradiation can effectively inhibit microbial growth, but the changes of lipid during irradiation have not been comprehensively analyzed in marble beef. Here, UHPLC-MS/MS was used to detect lipids changes of irradiated marble beef. A total of 1032 lipids were identified and classified into 3 lipid classes and 8 subclasses in irradiated marble beef. 9 lipid biomarkers were screened with increasing irradiation dose. 122 differential lipids were generated and involved in 4 metabolic pathways included Glycerophospholipid metabolism, Linoleic acid metabolism, alpha-Linolenic acid metabolism and Arachidonic acid metabolism though PC(18:0/14:0), PE(16:0/16:0) and PE(18:0/16:0) in irradiated. Our results showed that irradiation had effect on the lipid of marbled beef, but the increase of irradiation dose from 2.5 kGy to 4.5 kGy had little effect on lipids. These results help us to understand the dynamic changes of irradiated meat lipids and lay a foundation for the application of irradiation in meat preservation.
电子束辐照能有效抑制微生物生长，但在大理石花纹牛肉中，辐照过程中脂质的变化尚未得到全面分析。在此，采用超高效液相色谱-串联质谱法（UHPLC-MS/MS）检测辐照大理石花纹牛肉的脂质变化。在辐照大理石花纹牛肉中，共鉴定出1032种脂质，并将其分为3类脂质和8个亚类。随着辐照剂量增加，筛选出9种脂质生物标志物。通过辐照后的PC(18:0/14:0)、PE(16:0/16:0)和PE(18:0/16:0)产生了122种差异脂质，涉及甘油磷脂代谢、亚油酸代谢、α-亚麻酸代谢和花生四烯酸代谢4条代谢途径。我们的结果表明，辐照对大理石花纹牛肉的脂质有影响，但辐照剂量从2.5千戈瑞增加到4.5千戈瑞对脂质的影响较小。这些结果有助于我们了解辐照肉类脂质的动态变化，并为辐照在肉类保鲜中的应用奠定基础。
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