Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, China.
Nat Prod Res. 2024 Jul;38(13):2272-2281. doi: 10.1080/14786419.2023.2172006. Epub 2023 Feb 1.
Sagittatoside B is one of the principal diglucosides in Herba Epimedii. In this work, an ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF-MS) was applied to the rapid analysis of sagittatoside B metabolites in rats after oral administration. A total number of 17 metabolites were detected or tentatively identified from rat plasma, bile, urine and feces. The major metabolic pathways of sagittatoside B in rats were hydrolysis, hydrogenation, hydroxylation, dehydrogenation, demethylation, decarbonylation and conjugation with glucuronic acid and different sugars. This work revealed the metabolism of sagittatoside B , and reported the characteristic metabolic reactions of sagittatoside B for the first time. This provided the basis for the further research and development of sagittatoside B, and also provided reference for the metabolism of active flavonoid compounds.
箭叶淫羊藿苷 B 是淫羊藿中的主要双糖苷之一。在这项工作中，采用超高效液相色谱/四极杆飞行时间质谱（UPLC/Q-TOF-MS）快速分析了口服给药后大鼠体内箭叶淫羊藿苷 B 的代谢产物。从大鼠血浆、胆汁、尿液和粪便中共检测或推测出 17 种代谢产物。箭叶淫羊藿苷 B 在大鼠体内的主要代谢途径为水解、氢化、羟基化、脱氢、去甲基化、脱羰基和与葡萄糖醛酸及不同糖结合。本研究揭示了箭叶淫羊藿苷 B 的代谢途径，并首次报道了箭叶淫羊藿苷 B 的特征代谢反应。这为箭叶淫羊藿苷 B 的进一步研究和开发提供了依据，也为活性黄酮类化合物的代谢提供了参考。
