通过免疫亲和和高压液相色谱法从钳蝎科蝎毒中纯化诱导挛缩的昆虫毒素
Purification of contracture-inducing insect toxins from Buthinae scorpion venoms by immunoaffinity and high pressure liquid chromatography.
作者信息
de Dianous S, Kopeyan C, Bahraoui E M, Rochat H
机构信息
Laboratoire de Biochimie, U.D.C., CNRS UA 1179, INSERM U 172, Faculté de Médecine Secteur Nord, Marseille, France.
出版信息
Toxicon. 1987;25(7):731-41. doi: 10.1016/0041-0101(87)90123-1.
A method of rapid and selective detection and purification of contracture-inducing insect toxins from Buthinae scorpion venoms is described in this paper. It consists of two main steps: the first one is specific as it uses immunoaffinity chromatography with antibodies against Androctonus australis Hector IT; the second, reverse phase high pressure liquid chromatography, allows the final separation of the different toxins present in each of the three venoms studied. Two, three and four insect toxins have been purified, respectively, from the venoms of Androctonus australis Hector, Buthus occitanus mardochei and Leiurus quinquestriatus quinquestriatus. This work demonstrates that, in Buthinae venoms, contracture-inducing insect toxins antigenically related to AaH IT, the first one purified, constitute the most important and, in some cases, the only toxins present.
本文描述了一种从钳蝎科蝎毒中快速、选择性地检测和纯化诱导挛缩昆虫毒素的方法。该方法包括两个主要步骤：第一步具有特异性，它使用针对澳毒蛛（Androctonus australis Hector）昆虫毒素（IT）的抗体进行免疫亲和层析；第二步是反相高压液相色谱，可对所研究的三种毒液中的不同毒素进行最终分离。分别从澳毒蛛、北非蛛蝎（Buthus occitanus mardochei）和以色列金蝎（Leiurus quinquestriatus quinquestriatus）的毒液中纯化出了两种、三种和四种昆虫毒素。这项研究表明，在钳蝎科毒液中，与最早纯化出的澳毒蛛昆虫毒素（AaH IT）具有抗原相关性的诱导挛缩昆虫毒素是最重要的毒素，在某些情况下，也是唯一存在的毒素。
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