Routine methods in toxicology and therapeutic drug monitoring by high performance liquid chromatography. II. A rapid microscale method for determination of chloramphenicol in blood and cerebrospinal fluid.

A highly sensitive, specific method for determining chloramphenicol levels in human blood plasma and cerebrospinal fluid is described. The method uses high performance liquid chromatography for the analysis, requires minimal quantities of patient specimen, and thus is suitable for use in newborn and pediatric patients. The method described in this article is specifically developed for routine use in laboratories engaged in therapeutic drug monitoring. It has advantages over other methods because it is less time consuming and can be used with commercially available controls.