Science Medical Center, Saratov State University, Saratov, Russia.
A.V. Zelmann Center for Neurobiology and Brain Rehabilitation, Skolkovo Institute of Science and Technology, Moscow, Russia.
J Biophotonics. 2023 Jun;16(6):e202200379. doi: 10.1002/jbio.202200379. Epub 2023 Feb 15.
Fluorescent photoconvertible materials and molecules have been successfully exploited as bioimaging markers and cell trackers. Recently, the novel fluorescent photoconvertible polymer markers have been developed that allow the long-term tracking of individual labeled cells. However, it is still necessary to study the functionality of this type of fluorescent labels for various operating conditions, in particular for commonly used discrete wavelength lasers. In this article, the photoconversion of fluorescent polymer labels with both pulsed and continuous-wave lasers with 532 nm-irradiation wavelength, and under different laser power densities were studied. The photoconversion process was described and its possible mechanism was proposed. The peculiarities of fluorescent polymer capsules performance as an aqueous suspension and as a single capsule were described. We performed the successful nondestructivity marker photoconversion inside RAW 264.7 monocyte/macrophage cells under continuous-wave laser with 532 nm-irradiation wavelength, showing prospects of these fluorescent markers for long-term live cell labeling.
荧光光致变色材料和分子已被成功用作生物成像标记物和细胞示踪剂。最近，开发了新型荧光光致变色聚合物标记物，可以长期跟踪单个标记细胞。然而，仍然有必要研究这种荧光标记物在各种工作条件下的功能，特别是对于常用的离散波长激光器。在本文中，研究了 532nm 辐照波长的脉冲和连续波激光器以及不同激光功率密度下的荧光聚合物标记的光致变色。描述了光致变色过程，并提出了可能的机制。描述了荧光聚合物胶囊作为水悬浮液和单个胶囊的性能特点。我们在连续波激光下（532nm 辐照波长）在 RAW 264.7 单核/巨噬细胞内成功地进行了标记物的非破坏性光致变色，这表明这些荧光标记物在长期活细胞标记方面具有广阔的应用前景。
