Sindeeva Olga A, Demina Polina A, Kozyreva Zhanna V, Terentyeva Daria A, Gusliakova Olga I, Muslimov Albert R, Sukhorukov Gleb B
Vladimir Zelman Center for Neurobiology and Brain Rehabilitation, Skoltech, 3 Nobel Str., 121205 Moscow, Russia.
Science Medical Center, Saratov State University, 83 Astrakhanskaya Str., 410012 Saratov, Russia.
Nanomaterials (Basel). 2024 Jul 17;14(14):1215. doi: 10.3390/nano14141215.
Reliable cell labeling and tracking techniques are imperative for elucidating the intricate and ambiguous interactions between mesenchymal stromal cells (MSCs) and tumors. Here, we explore fluorescent photoconvertible nanoengineered vesicles to study mMSC migration in brain tumors. These 3 μm sized vesicles made of carbon nanoparticles, Rhodamine B (RhB), and polyelectrolytes are readily internalized by cells. The dye undergoes photoconversion under 561 nm laser exposure with a fluorescence blue shift upon demand. The optimal laser irradiation duration for photoconversion was 0.4 ms, which provided a maximal blue shift of the fluorescent signal label without excessive laser exposure on cells. Vesicles modified with an extra polymer layer demonstrated enhanced intracellular uptake without remarkable effects on cell viability, motility, or proliferation. The optimal ratio of 20 vesicles per mMSC was determined. Moreover, the migration of individual mMSCs within 2D and 3D glioblastoma cell (EPNT-5) colonies over 2 days and in vivo tumor settings over 7 days were traced. Our study provides a robust nanocomposite platform for investigating MSC-tumor dynamics and offers insights into envisaged therapeutic strategies. Photoconvertible vesicles also present an indispensable tool for studying complex fundamental processes of cell-cell interactions for a wide range of problems in biomedicine.
可靠的细胞标记和追踪技术对于阐明间充质基质细胞（MSC）与肿瘤之间复杂且模糊的相互作用至关重要。在此，我们探索荧光光转换纳米工程囊泡以研究间充质基质细胞在脑肿瘤中的迁移。这些由碳纳米颗粒、罗丹明B（RhB）和聚电解质制成的3μm大小的囊泡很容易被细胞内化。该染料在561nm激光照射下发生光转换，荧光按需发生蓝移。光转换的最佳激光照射持续时间为0.4ms，这在不对细胞进行过度激光照射的情况下提供了荧光信号标记的最大蓝移。用额外聚合物层修饰的囊泡显示出增强的细胞内摄取，而对细胞活力、运动性或增殖没有显著影响。确定了每个间充质基质细胞20个囊泡的最佳比例。此外，追踪了单个间充质基质细胞在二维和三维胶质母细胞瘤细胞（EPNT - 5）集落中2天内以及在体内肿瘤环境中7天内的迁移情况。我们的研究为研究间充质基质细胞 - 肿瘤动态提供了一个强大的纳米复合平台，并为设想的治疗策略提供了见解。光转换囊泡也是研究生物医学中广泛问题的细胞 - 细胞相互作用复杂基本过程的不可或缺的工具。
