Expression of FMD virus-like particles in yeast and immunogenicity of combine with CpG and aluminum adjuvant.

BACKGROUND

Inactivated vaccines are limited in preventing foot-and-mouth disease (FMD) due to safety problems. Recombinant virus-like particles (VLPs) are an excellent candidate for a novel vaccine for preventing FMD, given that VLPs have similar immunogenicity as natural viruses and are replication- and infection-incompetent.

OBJECTIVES

The 3C protease and P1 polyprotein of type O FMD virus (FDMV) was expressed in yeast to generate self-resembling VLPs, and the potential of recombinant VLPs as an FMD vaccine was evaluated.

METHODS

BALB/c mice were immunized with recombinant purified VLPs using CpG oligodeoxynucleotide and aluminum hydroxide gel as an adjuvant. Cytokines and lymphocytes from serum and spleen were analyzed by enzyme-linked immunosorbent assay, enzyme-linked immunospot assay, and flow cytometry.

RESULTS

The VLPs of FMD were purified successfully from yeast protein with a diameter of approximately 25 nm. The immunization of mice showed that animals produced high levels of FMDV antibodies and a higher level of antibodies for a longer time. In addition, higher levels of interferon-γ and CD4 T cells were observed in mice immunized with VLPs.

CONCLUSIONS

The expression of VLPs of FMD in provides a novel strategy for the generation of the FMDV vaccine.