Retrospective Study of the Epidemiology of Infection in the Neurosurgery Department of a Tertiary Hospital in China.

BACKGROUND

Although the epidemiology of is important, few studies examining transmission of have been reported, especially in wards with low detection rates, such as neurosurgery departments.

PURPOSE

This retrospective study investigated the epidemiology of infection in a neurosurgery department over a 24-month period, particularly examining the transmission of using whole-genome sequencing (WGS).

METHODS

strains were isolated and identified from fecal samples of neurosurgical patients. Toxigenic strains were typed using multilocus sequence typing, PCR ribotyping and using capillary gel electrophoresis. WGS was used to characterize ST-37/RT017 isolates, and comparative genomic analyses were performed to compare genomic differences between all ST-37 strains from other wards. The susceptibility to 8 antimicrobial agents was examined using the E-test.

RESULTS

Comparative genomic analyses revealed that isolates obtained from neurosurgical patients clustered into two lineages. Only strains s11052403 and s10090304, respectively, isolated from a patient on the 8th floor of the neurosurgery ward and a patient on the 9th floor, were highly similar, exhibiting differences of only two single-nucleotide polymorphisms. All ST-37/RT017 strains isolated from neurosurgical patients were resistant to multiple classes of antibiotics.

CONCLUSION

There is an urgent need to raise awareness of infection, and epidemiologic surveillance is required to detect clustering and transmission of cases in China. Strict disinfection of the environment is essential to reduce transmission of and achieve effective infection control in the hospital setting.