GidA在转录后水平调节过氧化氢酶的表达，并在毒力方面发挥作用。
GidA modulates the expression of catalases at the posttranscriptional level and plays a role in virulence.
作者信息
Srimahaeak Thanyaporn, Thongdee Narumon, Chittrakanwong Jurairat, Atichartpongkul Sopapan, Jaroensuk Juthamas, Phatinuwat Kamonwan, Phaonakrop Narumon, Jaresitthikunchai Janthima, Roytrakul Sittiruk, Mongkolsuk Skorn, Fuangthong Mayuree
机构信息
Program in Applied Biological Sciences, Chulabhorn Graduate Institute, Bangkok, Thailand.
Department of Biotechnology, Faculty of Engineering and Industrial Technology, Silpakorn University, Sanamchandra Palace Campus, Nakhon Pathom, Thailand.
出版信息
Front Microbiol. 2023 Jan 16;13:1079710. doi: 10.3389/fmicb.2022.1079710. eCollection 2022.
, which encodes a putative tRNA-modifying enzyme, is associated with a variety of virulence phenotypes. Here, we demonstrated that is responsible for the modifications of uridine in tRNAs . Loss of was found to have no impact on the mRNA levels of and , but it decreased KatA and KatB protein levels, resulting in decreased total catalase activity and a hydrogen peroxide-sensitive phenotype. Furthermore, was found to affect flagella-mediated motility and biofilm formation; and it was required for the full virulence of in both and macrophage models. Together, these observations reveal the posttranscriptional impact of on the oxidative stress response, highlight the complexity of catalase gene expression regulation, and further support the involvement of in the virulence of .
其编码一种假定的tRNA修饰酶，与多种毒力表型相关。在此，我们证明了……负责tRNAs中尿苷的修饰。发现……的缺失对……和……的mRNA水平没有影响，但它降低了KatA和KatB蛋白水平，导致总过氧化氢酶活性降低和对过氧化氢敏感的表型。此外，发现……影响鞭毛介导的运动性和生物膜形成；并且在……和巨噬细胞模型中，……的完全毒力都需要它。总之，这些观察结果揭示了……对氧化应激反应的转录后影响，突出了过氧化氢酶基因表达调控的复杂性，并进一步支持了……参与……的毒力过程。 （注：原文中部分关键信息缺失，用“……”代替，以便完整展示句子结构）
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