Department of Chemistry and Biochemistry, Northern Illinois University, DeKalb, IL 60115, USA.
Nucleic Acids Res. 2023 Feb 28;51(4):1600-1607. doi: 10.1093/nar/gkad031.
Discovery of oxidative catalysis with G-quadruplex•hemin constructs prompted a range of exciting developments in the field of biosensor design. Thus, G-quadruplex based DNAzymes with peroxidase activity found a niche as signal transduction modules in a wide range of analytical applications. The ability of nucleic acid scaffolds to recognise a variety of practically meaningful markers and to translate the recognition events into conformational changes powers numerous sensor design possibilities. In this work, we establish a catalase activity of G-quadruplex•hemin scaffolds. Catalase activated hydrogen peroxide decomposition generates molecular oxygen that forms bubbles. Observation of bubbles is a truly equipment free signal readout platform that is highly desirable in limited resources or do-it-yourself environments. We take a preliminary insight into a G-quadruplex structure-folding topology-catalase activity correlation and establish efficient operating conditions. Further, we demonstrate the platform's potential as a signal transduction modality for reporting on biomolecular recognition using an oligonucleotide as a proof-of-concept target. Ultimately, activatable catalases based on G-quadruplex•hemin scaffolds promise to become valuable contributors towards accessible molecular diagnostics applications.
具有 G-四链体·血红素结构的氧化催化作用的发现，促使生物传感器设计领域取得了一系列令人兴奋的进展。因此，具有过氧化物酶活性的基于 G-四链体的 DNA 酶作为信号转导模块，在各种分析应用中找到了自己的位置。核酸支架能够识别各种具有实际意义的标记物，并将识别事件转化为构象变化，这为众多传感器设计提供了可能性。在本工作中，我们建立了 G-四链体·血红素支架的过氧化氢酶活性。过氧化氢酶激活后会分解生成分子氧，从而形成气泡。观察气泡是一种真正无需设备的信号读出平台，在资源有限或 DIY 环境中非常理想。我们初步探讨了 G-四链体结构折叠拓扑-过氧化氢酶活性之间的关系，并确定了有效的操作条件。此外，我们还展示了该平台作为一种信号转导模式，用于报告基于寡核苷酸的生物分子识别的潜力，作为概念验证的目标。最终，基于 G-四链体·血红素支架的可激活过氧化氢酶有望成为可及分子诊断应用的有价值的贡献者。
